target amplification

target amplification

(1) A generic term for any method used to increase the copy number of a molecular species. The gold standard for target amplification is polymerase chain reaction; non-PCR methods include transcription-mediated amplification and nucleic acid sequence-based amplification.

(2) A generic term for any method used to identify a molecular species by increasing the number of target molecules, which includes:
   • Hybridisation to species-specific probes that are immobilised on membranes, microtiter plates or are in solution;
   • Analysis of RFLPs of amplified target genes;
   • Direct sequencing of PCR-amplified gene segments.
References in periodicals archive ?
Common complications include primer-dimer formation--which consumes reagents and reduces target amplification and signal levels--and misamplification of very similar, off-target sequences.
Amped's high sensitivity and specificity will enable the direct detection of pathogens present in clinical samples, even when present in small amounts, without requiring polymerase chain reaction-based target amplification, and when implemented in future tests will make it possible for Great Basin to provide PCR-like limits of detection at greatly reduced test times for certain targets of interest.
5) assay is a reverse-transcription PCR-based, target amplification assay targeting the gag p24 region of the genome.
In the cycles that follow, the UniPrimer anneals to the tag and directs the target amplification.
Its assays are based on its STAR (Scalable Target Amplification Routine) technology.
T] value represents the number of cycles required for target amplification to reach the exponential phase and is therefore inversely related to initial target concentrations.
The kits incorporate XTRANA's solid-phase nucleic acid extraction matrix, which provides for target amplification directly from the nucleic acid bound to the solid phase surface.
Because the target DNA or RNA may be present in very small amounts in clinical specimens, various signal amplification and target amplification techniques have been used to detect infectious agents in clinical diagnostic laboratories (5,6).
The combined technologies have the potential to provide a highly sensitive assay platform for SNP detection on any instrument systems with fluorescence or chemilumiscence detection capabilities without target amplification.
Relative to the microarray products provided by Affymetrix (AFFX), Agilent (A), Combimatrix (CBMX), and by Luminex (LMNX), Akonni's TruArray tests significantly simplify microarray workflows by combining conventional target amplification, fragmentation and labeling processes into a single tube or microfluidic chamber.
Target amplification has resulted in tests of exquisite sensitivity, with certain tests being capable of detecting less than a single organism.
19 November 2014 - South Korean molecular diagnostic tests developer Seegene Inc (KOSDAQ:096530) said Wednesday it had received a US patent protecting its tagging oligonucleotide capture and extension (TOCE) technology, allowing highly multiplexed target amplification and identification in a single tube.

Full browser ?