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At admission to the emergency service, a 5 mL urine sample was collected in an assay tube and mixed with 5 ml of 3% sulfosalicylic acid.
The sulfosalicylic acid test is useful for a quick diagnosis of proteinuria.
Contrariwise, sulfosalicylic acid test can be performed in random urine specimens obtained at admission.
In the final procedure, sample preparation before injection involved protein precipitation with 100 g/L sulfosalicylic acid dissolved in water and acetonitrile.
After 4 min, the Valco valve position was again switched to allow the extraction column to be purged by sulfosalicylic acid (1 g/L) and methanol and reequilibrated at a flow rate of 3 mL/min with water/acetic acid/methanol (92:3:5 by volume).
The results indicated that a precipitation with acetonitrile and sulfosalicylic acid achieved the optimal extraction recovery of analytes.
At the end of the incubation period, the reactions were terminated by adding 5 mL of sulfosalicylic acid at 60 mL [L.
Shephard and Whiting (17) compared four protein precipitants--BC, BTC, trichloroacetic acid, and sulfosalicylic acid for nephelometry--and found that BC had good precision and agreed most closely with the trichloroacetic acid-Ponceau S dye-binding method.
The method of Andersson and co-workers (16,18) also requires special handling and rapid processing of the sample, along with the addition of sulfosalicylic acid to the separated EDTA plasma.
Most laboratories quantify urine protein with a precipitation method, such as trichloroacetic or sulfosalicylic acid, or with a protein binding dye such as Coomassie blue or Ponceau S.
After incubation, protein precipitation was achieved by the addition of 100 [micro]L of 150 g/L sulfosalicylic acid.
75, containing 6 mmol/L OSA and 100 mL/L methanol was used, sulfosalicylic acid was not retained by the column.

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