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subcloning

   Also found in: Wikipedia 0.01 sec.
subcloning
a restriction fragment of an original DNA that has been cloned may be further digested with another restriction enzyme and the smaller fragments cloned.


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To determine which parts of the Lycopersicon mt genomes are involved in the recombination events I am subcloning polymorphic bands from the mtRAPD profiles and comparing to known plant mt sequences.
For construction of the Gal4 DBD-PXR ligand binding domain (LBD) fusion plasmid (Gal-PXR), PCR was used to amplify LBD-specific fragments of rat PXR; exonuclease III-mediated subcloning (Li and Evans 1997) was used to clone the fragments between the EcoRI and BamHI sites of pCMX-Gal4.
 
 
 
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