Thereafter, the technology formed the basis of a NewCo which raised funding to develop the technology further and take it to the market place (initially focussed on making a magnetic streptavidin
This Global Report 2015 is a result of industry experts' diligent work on researching the world market of streptavidin
The plates were coated with 100 pl of Streptavidin
(5 [micro]g/ml) per plate.
The DNA fragments hybridized to biotinylated probes were selectively captured by streptavidin
coated magnetic beads (Streptavidin
Magnesphere Paramagnetic Particles, Promega, Shanghai, China).
Examples of tags meeting these criteria include fluorescein paired to anti-fluorescein antibodies, and biotin paired to streptavidin
The leftover, unbound polymer is then added to the etalons, where the corresponding colour change indicates how much polymer is left, and in turn, how much streptavidin
there was to bind with.
In this application, QDs tagged with streptavidin
are employed in conjunction with biotin-tagged bacteriophage to successfully detect as little as 10 contaminating cells per mL in an aqueous sample (30).
is also employed in Siemens Immulite (Siemens AG, Erlangen, Germany) specific-allergy testing.
Then, due to the strong interaction of streptavidin
and biotin, the enzyme containing streptavidin
is attached to the biotinylated monolayer of olioligonucleotide that has been attached to the DNA molecule on the surface," Dr.
The presence of targets was detected using a dye-labeled protein, Cy3-labeled streptavidin
, and measuring the fluorescence signal.
The sample was adsorbed on uncoated microparticles and microparticles coated with streptavidin
prior to vitamin D assay.
Once hybridization of these various components has been completed, the biotin-oligo(dT) probes (and the attached transcripts) are affinity captured by streptavidin
coated onto magnetic beads.