Researchers found that microbubbles conjugated to streptavidin
can be coupled to the surface of a cell, where mechanosensitive Piezo1 ion channels are expressed.
Once this bridge is formed, streptavidin
peroxidase attached to the colorogenic substrate (TMB) is bound.
Louis, MO, USA), MG-l32 from Enzo Life Sciences (Farmingdale, NY, USA), Cy5-conjugated Streptavidin
from Jackson ImmunoResearch Laboratories (West Grove, PA, USA), and Coomassie Stain Solution from Bio-Rad (Hercules, CA, USA).
This method employed a mouse monoclonal antibody (NCL-COX-2) of Vision Biosystems, NovocastraTM as primary antibody and secondary antibody of Ultra Tech HRP (DAB) Streptavidin
Biotin Detection System from Beckman Coulter was used.
Possible solutions may require removal of biotin in the assay design by pretreating samples with streptavidin
or by adding biotin extraction steps.
Four subprojects have been tailored to address the challenges: i) knock-out deleterious components present in the periplasm; ii) improve the cofactor uptake through the outer-membrane; iii) engineer streptavidin
to boost the AMs performance; and iv) rely both on screening and selection strategies to evolve AMs in vivo.
Thermo Scientific has launched its next-generation MSIA Streptavidin
EVO microcolumns for large molecule bioanalysis.
Blots were incubated with HRP-conjugated anti-rabbit secondary antibodies (1:5,000, Southern Biotech) or HRP-conjugated streptavidin
(1:40,000, Invitrogen) for 1 hr at 25[degrees]C.
The money enables Quad to launch its MagCloudz Streptavidin
Cell Separation Kit, a commercially available product incorporating QuickGel technology.
The plates were coated with 100 pl of Streptavidin
(5 [micro]g/ml) per plate.
The DNA fragments hybridized to biotinylated probes were selectively captured by streptavidin
coated magnetic beads (Streptavidin
Magnesphere Paramagnetic Particles, Promega, Shanghai, China).
The leftover, unbound polymer is then added to the etalons, where the corresponding colour change indicates how much polymer is left, and in turn, how much streptavidin
there was to bind with.