single-cell gel electrophoresis

single-cell gel electrophoresis

a type of gel electrophoresis used to detect the genotoxic potential of environmental hazards, such as radiation, heavy metals, and toxic chemicals. Such agents may cause breaks in the nuclear DNA of cells. When such a cell is lysed and exposed to electrophoresis that denatures its DNA, the damaged DNA moves toward the electric field, making a formation like the tail of a comet. Also called comet assay.
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Assessment of the pharmacological effect of silymarin on ethanol-induced DNA damage by single-cell gel electrophoresis.
Alkaline single-cell gel electrophoresis (comet assay): a simple technique to show genomic instability in sporadic breast cancer.
Single-cell gel electrophoresis (comet assay): methodology, potential applications, and limitations in cancer research.
They examined the genotoxicity of silica, titanium dioxide, polystyrine and C60 fullerene nanoparticle suspensions using the alkaline single-cell gel electrophoresis assay (Comet assay) to quantify breaks in single and double stranded DNA.
DNA damage products have been identified and quantified by a wide range of analytical techniques, such as single-cell gel electrophoresis, '2P- postlabeling, immunoassay, gas chromatography/mass spectrometry, high performance liquid chromatography, and electrochemical and electrochemiluminescence sensors [7-9].
The in vitro genotoxicity of imazalil and thiabendazole fungicides and the insecticide chlorpyrifos, compounds used in Costa Rican banana plantations, was evaluated with the single-cell gel electrophoresis technique (comet assay).
Key words: Single-cell gel electrophoresis, comet assay, imazalil, chlorpyrifos, thiabendazole, lymphocytes, genotoxic.
We observed that DMSO and CAT basically abolished most of the As-induced DNA single strand breaks (SSBs) in human cells with single-cell gel electrophoresis (SCGE) assay (5).

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