single-cell gel electrophoresis

single-cell gel electrophoresis

a type of gel electrophoresis used to detect the genotoxic potential of environmental hazards, such as radiation, heavy metals, and toxic chemicals. Such agents may cause breaks in the nuclear DNA of cells. When such a cell is lysed and exposed to electrophoresis that denatures its DNA, the damaged DNA moves toward the electric field, making a formation like the tail of a comet. Also called comet assay.
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The exact laboratory technique used was the COMET assay, otherwise known as Single-Cell Gel Electrophoresis (SCGE), which is a very sensitive and well-known peer accepted laboratory method for assessing damage to cellular DNA.
We observed that DMSO and CAT basically abolished most of the As-induced DNA single strand breaks (SSBs) in human cells with single-cell gel electrophoresis (SCGE) assay (5).

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