Obesity-induced gut microbial metabolite promotes liver cancer through senescence secretome
proteins characteristically have signal cleavage peptides that permit their active export across the plasmalemma.
Identification of head and neck squamous cell carcinoma biomarker candidates through proteomic analysis of cancer cell secretome
Nine specific proteins have been identified as candidate biomarkers, including lipocalin-1, which has demonstrated increased expression in aneuploid blastocyst secretome
, the material secreted from the embryo.
Although, cellular senescence has been considered as a natural mechanism to combat uncontrolled cell growth or cancer, some cell types express a secretome
asinina mantle secretome
reveals that the genome encodes a large diversity of novel, secreted proteins (Jackson et al.
The adipokinome (which together with lipid moieties released, such as fatty acids and prostaglandins, constitute the secretome
of fat cells) includes proteins involved in lipid metabolism, insulin sensitivity, the alternative complement system, vascular haemostasis, blood pressure regulation and angiogenesis, as well as the regulation of energy balance.
75,77) An extensive analysis of the pancreatic cancer secretome
, by stable isotope labeling with amino acids in cell culture, identified 145 proteins differentially secreted by pancreatic cancer cells as compared to normal pancreatic ductal cells.
A sampling of topics: two-dimensional polyacrylamide gel electrophoresis for cardiovascular proteomics, subcellular fractionation, optimization of cardiac troponin I pull-down by IDM affinity beads and SELDI, characterization of the human atheroma plaque secretome
by proteomic analysis, the proteome of endothelial cells, the proteome and secretome
of human arterial smooth muscle cell, isolation of the platelet releasate, cardiovascular biomarker discover by SELDI-TOF mass spectrometry, and techniques to decipher molecular diversity by phage display.
Reduced genomic potential for secreted plant cell-wall-degrading enzymes in the ectomycorrhizal fungus Amanita bisporigera, based on the secretome
of Trichoderma reesei.
An example is the use of data generated by the NCI's The Cancer Genome Atlas to guide the discovery of ovarian cancer-related secretome
genes that can be further prioritized and subjected to scrutiny for clinical validation.
More recently, a study on the gene expression in the mantle of vetigastropod Haliotis asinina has revealed a complex secretome
and characterized a number of genes involved in shell construction and coloration in this tropical abalone (Jackson et al.