scanning optical microscopy

scanning optical microscopy

A method of light microscopy in which the object, often a block of wet or dry tissue, is illuminated with a scanned laser beam in such a way as to bring a narrow plane into focus while leaving the remainder out of focus. Serial observations, with photographs, of different planes can be made so that cutting sections is unnecessary. A three-dimensional image can be built up. The sites of binding of fluorescence-labelled antibodies can be determined with great accuracy.
References in periodicals archive ?
in addition to others such as scanning tunneling microscopy (STM), near-field scanning optical microscopy (NSOM or SNOM, depending on the continent), and other lithographic methods.
Hafner, "Polarization mode coupling due to metal-layer modifications in apertureless near-field scanning optical microscopy probes," J.
Chichester, "Single molecules observed by near-field scanning optical microscopy," Science, Vol.
Dunn, "Probing single molecule orientations in model lipid membranes with near-field scanning optical microscopy," J.
Laser scanning optical microscopy has been used in cell imaging and other biological applications since the early 1980s.
Hemel Hempstead, UK, has been at the forefront of developments in laser scanning optical microscopy and has introduced several major innovations in the area.
The new device grows out of a method known as near-field scanning optical microscopy (NSOM), which is widely used for imaging.
Park Systems, one of the earliest manufacturers of AFMs, produces AFMs and SPMs for both small- and large-sample measurements, Near-field Scanning Optical Microscopy (NSOM), Raman Spectrometry, and for industrial applications, including hard disk inspection, next-generation sliders, sidewall/overhang imaging and profiling, and semiconductors.
Near-field scanning optical microscopy (NSOM) is an interesting technique that surmounts one of the limitations of ordinary light microscopy: the diffraction limit.
Near-field scanning optical microscopy (NSOM) uses an optical fiber drawn to a fine tip.
The company's products are based on its core competencies in high precision measurement, including interferometry, confocal scanning optical microscopy, application specific vision metrology, atomic force microscopy, systems integration, automated piece parts handling, and precision optical components.
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