sandwich technique

sandwich technique

a method of identifying antibodies or antibody-synthesizing cells in a tissue preparation. A solution containing a specific antigen is applied to the preparation. If antibodies to the antigen are present in the tissue, they will bind to the antigen. Unbound antigen is washed away, and then a fluorochrome-labeled antibody specific for the antigen is added. The result is a complex of antigen sandwiched between antibodies, which can be detected by fluorescence microscopy.
References in periodicals archive ?
Serum quantitative assays: The principle of the modern laboratory hCG immunoassay is based on the sandwich technique whereby a capture antibody binds one site on hCG, immobilizing it and a signaling antibody--labeled with an enzyme (EIA) or with radioactive iodine (RIA)--binding a distant site on hCG, labeling the immobilized complex.
The sandwich technique is so-called because of the layered applications of gelfoam and coil used to close the access site.
IMPLEMENT THE SANDWICH TECHNIQUE IN YOUR COACHING STYLE.
One proven technique for effective delivery of criticism is the Sandwich Technique (ST).
However, the Sandwich Technique is a proven method for increasing your athletes' acceptance of criticism.
One manager who uses this sandwich technique describes another method that's proven helpful.
The container, which is manufactured using an injection moulded sandwich technique to give strength and durability, is designed to carry a flexible reusable liner bag, which is placed inside the IBC and filled with the liquid.
An increased use of the sandwich technique should be favored by a better understanding of the mold filling process and the relation between molding parameters, material distribution and mechanical properties.
There are limitations to the sandwich technique employed here, for example the loss of toluene from the inner sandwich layer during the heat-sealing process, large standards of deviation for peak areas from duplicate samples, and the need for data adjustments to account for water volume loss during sampling.
In some cases, sandwich techniques have already led to improved sensitivity, while use of two monoclonal antibodies directed at different antigenic sites on a single molecule--himan chorionic gonadotropin, for example--has led to greatly improved specificity.