sandwich technique

sandwich technique

a method of identifying antibodies or antibody-synthesizing cells in a tissue preparation. A solution containing a specific antigen is applied to the preparation. If antibodies to the antigen are present in the tissue, they will bind to the antigen. Unbound antigen is washed away, and then a fluorochrome-labeled antibody specific for the antigen is added. The result is a complex of antigen sandwiched between antibodies, which can be detected by fluorescence microscopy.
References in periodicals archive ?
Standardized scenarios based on various feedback situations and corresponding checklists can be synthesized following any of the available effective feedback models or strategies such as Pendleton's model or sandwich technique, in which a praise-critique-praise approach is used to allow a holistic coverage of major issues pertaining to feedback practices (Table-I).
The IV3000 sandwich technique for fingertip injury is a non-surgical alternative to the standard skin grafts or flaps used by hand surgeons for distal fingertip injuries.
In an initial set of experiments reminiscent of a peanut butter-and-jelly sandwich technique, researchers took stem cells from the colons of babies undergoing intestinal surgeries and from mice, then added immune cells called macrophages, the body's scavengers that seek out and engulf debris along with foreign and diseased cells.
62% dentists adopted sandwich technique using RMGIC, 60% used GIC in sandwich restoration with immediate composite placement, 39% used flowable composite as a gingival increment, 4.
Hydro procedures were then performed and the nucleus prolapsed into the anterior chamber and lens expression was then performed by the sandwich technique.
Serum quantitative assays: The principle of the modern laboratory hCG immunoassay is based on the sandwich technique whereby a capture antibody binds one site on hCG, immobilizing it and a signaling antibody--labeled with an enzyme (EIA) or with radioactive iodine (RIA)--binding a distant site on hCG, labeling the immobilized complex.
Next time, try reader James Nelson's sacrificial plywood sandwich technique.
IMPLEMENT THE SANDWICH TECHNIQUE IN YOUR COACHING STYLE.
One manager who uses this sandwich technique describes another method that's proven helpful.
The container, which is manufactured using an injection moulded sandwich technique to give strength and durability, is designed to carry a flexible reusable liner bag, which is placed inside the IBC and filled with the liquid.
An increased use of the sandwich technique should be favored by a better understanding of the mold filling process and the relation between molding parameters, material distribution and mechanical properties.