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sandwich technique |
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sandwich technique, a method of identifying antibodies or antibody-synthesizing cells in a tissue preparation. A solution containing a specific antigen is applied to the preparation. If antibodies to the antigen are present in the tissue, they will bind to the antigen. Unbound antigen is washed away, and then a fluorochrome-labeled antibody specific for the antigen is added. The result is a complex of antigen sandwiched between antibodies, which can be detected by fluorescence microscopy. How to thank TFD for its existence? Tell a friend about us, add a link to this page, add the site to iGoogle, or visit webmaster's page for free fun content. |
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