sandwich immunoassay


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Related to sandwich immunoassay: Enzyme immunoassay

sandwich immunoassay

An immunoassay in which the analyte is bound to a solid phase and a labeled reagent subsequently bound immunochemically to the analyte.
See also: immunoassay
References in periodicals archive ?
On receiving the results, the performance of the sandwich immunoassay with FF beads was compared with conventional immunoassays.
LIMIT OF DETECTION, LIMIT OF QUANTIFICATION, AND REPRODUCIBILITY IN THE SANDWICH IMMUNOASSAY WITH FF BEADS
Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay.
Toward the limits of sandwich immunoassay of very low molecular weight molecules.
Plasma concentrations of sGPVI were determined by use of a bead-based sandwich immunoassay and mAb 8E9 reactive with human GPVI as capture antibody.
These 3 samples were diluted 50-fold to match the working range of the sandwich immunoassay.
We showed that MAbs specific to fragment 28-56, when used in sandwich immunoassay, detected only aminor portion (about 10%) of endogenous NTproBNP measured by assay [15C4.
We determined that our sandwich immunoassay for the measurement of copeptin has a functional assay sensitivity (defined as the lowest value with an interassay CV <20%) of 2.
In this study, commercial anticTnI MAbs specific to different epitopes were used as capture antibodies in sandwich immunoassays with an anti-troponin C MAb used as a tracer.
Additionally, sandwich immunoassays require 2 validated antibodies for each constituent protein.
Most commercially available immunological methods for the detection of bacteria are based on conventional sandwich immunoassays, which involve the formation of an immunocomplex with an immobilized antibody, the target bacteria, a second labeled antibody, and the separation of free and bound antibody fractions by washing steps.
To address these challenges, we have developed a new bioanalytical system composed of a disposable microfluidic compact disc (CD) device (Gyrolab Bioaffy[TM]) that is intended for sandwich immunoassays and an instrument for automatic processing of CDs (Gyrolab Workstation).