Caption: Figure 2--Photomicrographs of the joint cartilage of mice after the experimental induction of OA in DMM: A, E), group treated with AuNP-PAH; A: slight unevenness of the joint surface (arrow) with disorganization of the chondrocyte columns; B, F) group treated with curcumin; C, G) group treated with AuNP-PAH-curcumin; C) Integrity of the joint surface can be seen despite the slight disorganization of chondrocyte columns and cluster formation (arrow); D, H) control group treated with saline solution; D) Intense fibrocartilage formation, disorganization, and bone remodelling; H) intense decrease in proteoglycans shown by the decrease in Safranin
O/Fast Green staining.
Visualization of cartilaginous nodules with Safranin
O and SEM confirmed that ASCCTRL underwent more effective chondrogenesis in comparison to control group.
The UF range was estimated from the tidemark to the furthest recognizable chondrocyte within the ligament, as described previously,11 and this area was stained in red through Safranin
O staining (Fig.
O staining showed a significantly different proteoglycan profile between the CON group and the injury groups.
Slices were mounted on glass slides and stained with Safranin
O (Sigma-Aldrich[R]) and fast green (Sigma[R]).
Sections were stained with Johansen's safranin
(Johansen 1940) and 0.
Eberhard, "Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin
technique with microwave heating," Journal of Clinical Microbiology, vol.
Free hand sections were clarified in 50% sodium hypochlorite, and stained with 1% safranin
Three specimens were identified as Cryptosporidium species using initial testing with auramine-rhodamine flouorescent stain and confirmatory testing with safranin
stain or on formalin, either concentrate or on a direct wet prep, from a sodium acetate formalin container.
Finally, the sections were stained with safranin
for 50 min, dehydrated and mounted in DPX.
Then the secondary stain, safranin
was added, and incubated for 1 min.
0 (SO) histopathological staining revealed that CLX and SMN improved the MIA-induced destruction and fibrillation in cartilage surface.