After 20 minutes of washing in 4 changes of distilled water, sections were counterstained lightly with safranin
, dehydrated, and mounted.
The joint sections were stained with Safranin
0 to determine the proteoglycan content in the cartilage matrix and serial sections were scored for the degree of chondrocyte death and cartilage surface erosion.
The proteoglycan area (PA) was obtained by measuring the red-stained area in five random standardised rectangular fields (200x200 [micro]m) in the fibrocartilage zone of a Safranin
O section (Figure 2).
The strain grew on Brucella agar supplemented with 5% horse serum in the presence of basic fuchsine, thionine, and growth on safranin
Tedavide kullanilan UDKA ve kenodeoksikolik asit, safraya kolesterol salgisini azaltarak safranin
kolesterol doygunlugunun dusmesine neden olmaktadir (22,23).
the sections were stained in 1% alcoholic safranin
(in 50% ethanol) and mounted in dilute glycerine.
In order to obtain a better resolution of structural details, staining of the fibers was performed by adding a drop of aqueous safranin
and then covering with a cover glass.
Finally, the slide is counterstained for 30 seconds with safranin
, rinsed, and air dried.
The researcher also stained prepared slides with Safranin
Biofilm formation was determined by safranin
staining and absorbance readings at 492 nm using a spectrophotometer.
The staining was executed with safranin
and fast green combination according to the procedures described by Purvis et al.
Sections, 10 [micro]m thick were made with a rotary microtome and double stained in safranin