Quant aux farines de commerce, elles contiennent du saccharose
plus sensible a l'hydrolyse enzymatique, ce qui favorise la liberation d'importantes quantites de sucres simples.
L5 utilization of carbohydrate substrates Carbohydrate Utilization Glycerol - Erythritol - D-arabinose - L-arabinose - Ribose + D-xylose - L-xylose - Adonitol - [beta]-Methyl-D-xyloside - Galactose - D-glucose + D-fructose - D-mannose - L-sorbose - Rhamnose - Dulcitol - Inositol - Mannitol - Sorbitol - [alpha]-Methyl-D-Mannoside - [alpha]-Methyl-D-Glucoside - N acetyl glucosamin - Amygdalin - Arbutin - Esculin + Salicin - Cellobiose - Maltose + Lactose - Melibiose + Saccharose
+ Trehalose - Inulin - Melezitose - D-raffinose + Amidon - Glycogen - Xylitol - [beta]-Gentiobiose - D-turanose - D-lyxose - D-tagatose - D-fucose - L-fucose - D-arabitol - L-Arabitol - Gluconate -(+) 2-keto-gluconate - 5-keto-gluconate - +: positive, -: negative.
apnea, septicemia, seizures, respiratory or cardiac insufficiency); diabetic ketoacidosis; Reye & Reye-like syndromes; increase with younger age; saccharose
, fructose, lactose; drugs inducing hyperlactemia; dialysis bath; MCT administration Lysine, glycine, serine metabolism Glutaconate Glycerate Uremia; increase with younger age Glycolate (83, 84) Ethylene glycol poisoning Glyoxylate 2-Hydroxyadipate (85) 3-Hydroxyglutarate (85) 2-Ketoadipate (85) Glutarate (18, 19, 85-87) 2-Ketoglutarate degradation; bacterial gut metabolism; uremia; ethylene glycol poisoning; lithium Oxalate (83, 88-90) Enteric malabsorption (regional enteritis or ileitis, celiac sprue disease, resection of ileum, Crohn disease); idiopathic stone disease; pyridoxine deficiency; increase with younger age; diet (e.
Before individual patient sera were frozen at or below -20[degrees]C, either 50 or 200 g/L saccharose was added to each individual serum aliquot that was intended for use in future pool preparation.
The final saccharose concentration in CRMs 1-3 was maintained at 50 g/L.
CRMs 7, 8, and 9, with low, medium, and high HDL-chol concentrations, respectively, and a final saccharose concentration of 200 g/L, were prepared from randomly gathered serum aliquots that were initially frozen with 200 g/L saccharose.
All pools were stored at -80[degrees]C, and none contained saccharose.
Red blood cells were lysed by the sequential addition of one volume of erythrocyte lysis buffer (320 mmol/L saccharose
, 5 mmol/L Mg[Cl.
PBS (300 [micro]L) containing saccharose
(100 g/L) was added to each well of the microplates, which were then incubated at room temperature for 1 h.
Initially, sera were analyzed after addition of increasing concentrations of saccharose (20, 75, 125, or 200 g/L) to produce samples characterized by an increase in background absorbance and a slight increase in viscosity.
Analyses of the samples containing 200 g/L saccharose with the first-generation precipitation methods frequently required ultrafiltration of the supernates.
5 PEG/Dex >200 ND ND (a) Precipitation was tested in two ways: by raising the background absorbance of the serum with saccharose (final concentrations 75, 125, or 200 g/L); and by assaying hypertriglyceridemic serum with triglyceride concentrations up to 54.