Other immunohistochemistry techniques include Avidin-Biotin Complex (ABC), Polymer based detection, tyramide or catalysed signal amplification, and rolling circle
The technique relies upon two target-specific primary antibodies, a "plus" and "minus" pair of species-specific PLA probes conjugated to DNA oligos that-when a target pair of proteins are in close proximity-can hybridize to trigger a rolling circle
The researchers used an RNA synthesis method known as rolling circle
transcription to produce extremely long strands of RNA made up of a repeating sequence of 21 nucleotides.
Signal amplification of padlock probes by rolling circle
Other topics include hybrid nanorods made from sequences of natural trimeric fibrous proteins using the fibritin trimerization motif, the Leucine zipper as a building block for self assembled protein fibers, biomimetic synthesis of biomorphic nanostructures, synthesis and primary characterization of self assembled peptide-based hydrogels,and the assembly of nanospecies on repetitive DNA sequences generated on gold nanoparticles by rolling circle
D] plan as a trajectory of a point which is fixed by the rolling circle
Several excellent chapters describe the uses of Phi29 DNA polymerase and of applications using isothermal rolling circle
Only five years old, rolling circle
amplification technology (RCAT) has become an important addition to many types of labs.
Using a technology called Rolling Circle
Amplification (RCA) and a DNA polymerase licensed exclusively to Amersham Pharmacia Biotech, the new TempliPhi method provides a cost-effective alternative to growing bacteria and purifying the template prior to sequencing.
The Friday Night Skate is a huge rolling circle
that gets started weekly at 10 p.
today announced a major breakthrough that should lead to an explosion in the applications of rolling circle
amplification (RCA) of nucleic acids in a wide range of diagnostic markets.
amplification can be applied as the 7th option for signal generation (Fig.