rocket immunoelectrophoresis


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immunoelectrophoresis

 [im″u-no-e-lek″tro-fo-re´sis]
a method of distinguishing proteins and other materials on the basis of their electrophoretic mobility and antigenic specificities.
rocket immunoelectrophoresis electrophoresis in which antigen migrates from a well through agar gel containing antiserum, forming cone-shaped (rocket) precipitin bands.

rock·et im·mu·no·e·lec·tro·pho·re·sis

a quantitative method for serum proteins that involves electrophoresis of antigen into a gel containing antibody; the technique is restricted to detection of antigens that move to the positive pole on electrophoresis. See: electroimmunodiffusion.

immunoelectrophoresis

separation, usually in an agar gel, of complex mixtures of antigens which then combine, following immunodiffusion, with antibody to form precipitation lines for each separated antigen.

counter immunoelectrophoresis
antigen and antibody are placed in separate wells, close together and electric current is applied first in one direction then in the other. When they migrate and meet, a line of precipitin is formed.
rocket immunoelectrophoresis
electrophoresis in which antigen migrates from a well through agar gel containing antiserum, forming cone-shaped (rocket) precipitin bands; the area under the cone is used to calculate the amount of antigen.
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