The AMP and ribose-5-phosphate
were not hydrolysed.
This may be done by the activation of reductive pentose phosphate pathway (PPP) by high-dose thiamine therapy that would increase transketolase (TK) activity and stimulate the conversion of glyceraldehyde-3-phosphate (GA3P) and fructose-6-phosphate (F6P) to ribose-5-phosphate
(R5P), thus reducing the risk of the development of diabetic complications.
The enzyme activity with other phosphorylated compounds such as adenosine 5'-triphosphate (ATP), adenosine 5'-monophosphate (AMP), ribose-5-phosphate
, glucose-6-phosphate and 2,3-bisphosphoglycerate (2,3-BPG) was measured under the standard assay condition using 1 mM of each substrate.
The pentose phosphate pathway (PPP) generates reducing equivalents in the form of nicotinamide adenine dinucleotide phosphate (NADPH) and ribose-5-phosphate
In this set of biochemical reactions, a molecule called glucose-6-phosphate, which is derived from the sugar glucose, is modified by transketolase, yielding two products--a sugar called ribose-5-phosphate
and a molecule called reduced nicotinamide adenine dinucleotide phosphate (NADPH) (see figure 2).