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reversed phase

    0.01 sec.
reversed phase,
a chromatographic mode in which the mobile phase is more polar than the stationary phase.


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HPLC methods applying the reversed phase technique, where the eluating buffer solution is supplemented by a large hydrophobic cationic, typically a tetrabutylammonium (TBA) compound, were probably first used for the separation of nucleotides by Juengling and Kammermeier (1980), who applied two separate isocreatic runs in different conditions to resolve Cr, PCr, and adenine nucleotides.
Young explained, "[it is] based on packed columns that use reversed phase columns operated at high pH, followed by low pH to fully exploit the ionic and hydrophobic structure of biomolecules.
Reversed phase high performance liquid chromatography analysis of ciprofloxacin and its different ophthalmic solutions including sunlight-exposed samples of eye drops were carried out using a Shimadzu LC-10 as pump, a Shimadzu SPD-10A UV detector, a Shimadzu C-R 6A integrator, and a Rhedyne-7125 injector.
 
 
 
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