High resolution reversed phase
high performance liquid chromatography of peptides and proteins.
The most likely technique applied will be ion-paired reversed phase
chromatography or hydrophilic interaction liquid chromatography.
With the ever-increasing complexity of pharmaceutical compounds, traditional purification processes including crystallization, and even normal or reversed phase
chromatography, are often too expensive, and, worse, ineffective," says Rene De Vaumas, Executive Vice President in charge of Novasep Synthesis' business development.
The first portion of the study examined the effect of mobile phase conditions on a reversed phase
19) from Roche Diagnostics, and the Jupiter[TM] Proteo column (C12 reversed phase
There are two main types of phase separation: (1) the normal phase HPLC which consists of a polar column material and relatively nonpolar solvents, and (2) reversed phase
HPLC which consists of a nonpolar column material and relatively polar solvents.
It offers LC media and columns for reversed phase
, ion exchange, hydrophobic interaction, gel filtration, and affinity separations and K-Prime 40 Biochromatography systems for process LC.
The supernatant was injected directly onto an HPLC consisting of a reversed phase
column (Prodigy C18, 250 x 4.
It can be employed with everything from hexane/isopropanol to methanol/water phases due to its intermediate polarity which is between that of the original silica gel and reversed phase
Supercritical fluid chromatography is a normal phase technique that provides different selectivity compared to reversed phase
Fractions from reversed phase
high-performance liquid chromatography showed strong antimicrobial activity against L.
This column design exhibits very high column efficiency, providing a stable, reversed phase
packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides.