reverse transcription


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re·verse tran·scrip·tion

reversal of the normal pattern of transcription (from DNA to RNA); the effective means is the viral enzyme reverse transcriptase.

reverse transcription

n.
The process by which DNA is synthesized from an RNA template by means of the enzyme reverse transcriptase.

reverse transcription

the process of synthesizing complementary DNA from an RNA TEMPLATE, by the ENZYME REVERSE TRANSCRIPTASE. A PRIMER is required for enzyme activity. The process tends to be error-prone, because there is no EDITING of the newly synthesized DNA and therefore MUTATIONS can accumulate. Reverse transcription is a reversal of the normal flow of genetic information from DNA to RNA.see CENTRAL DOGMA.

transcription

the synthesis of an RNA copy from a nucleotide sequence in a limited region of DNA. See also deoxyribonucleic acid and transcriptase.

transcription-control region
the cis-acting DNA sequences regulating transcription of a specific gene.
transcription factor (TF)
a general term for any protein, other than RNA polymerase, required to initiate or regulate transcription in eukaryotic cells. General TFs are involved in the formation of the transcription-preinitiation complexes near the start site and are required for transcription of all genes; specific TFs stimulate (activators) or inhibit (suppressors) transcription of particular genes by binding to their regulatory sequences.
nested set transcription
a hallmark of the replication strategy of coronaviruses, toroviruses and arteriviruses, in which a nested set of subgenomic mRNAs are generated having identical 5′-leader sequences and a common 3′-termini, but because of discontinuous transcription each mRNA has a unique coding sequence and is transcribed into a unique viral protein.
reverse transcription
the synthesis of a DNA copy from a RNA template, catalyzed by reverse transcriptase.
transcription unit
see operon.
References in periodicals archive ?
Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription.
Number of newly febrile horses and results of RT-PCR and a virus neutralization test, Japan, 2014 * Sep 1-7, Sep 8-14, Sep 15-21, Sep 22-28, Test n = 3 n = 3 n = 12 n = 15 RT-PCR NT NT NT 4/5 ([dagger]) Neutralization NT NT 4/4 ([double 4/4 dagger]) Sep 29-Oct 5, Oct 6-12, Oct 13-19, Test n = 13 n = 16 n = 7 RT-PCR 11/13 6/16 3/7 Neutralization 5/5 3/6 NT Oct 20-26, Oct 27-Nov Test n = 2 2, n = 6 RT-PCR 1/2 0/6 Neutralization NT NT * NT, not tested; RT-PCR, reverse transcription PCR.
Lys](UUU) in the presence of thymine during reverse transcription (Fig.
5 units of Taq DNA Polymerase, 6 [micro]L of the reverse transcription reaction described above, and 20 pmol each of CYP1A1 primers.
In RNA-based research applications, the use of reverse transcription is critical to the real-time PCR workflow.
Reverse transcription was performed by using the Superscript II RNase H-reverse transcription procedure (Invitrogen, Carlsbad, CA, USA) and primer TX30SXN as described (14).
Laboratory diagnostic techniques used included viral isolation, hemagglutinin inhibition serology, and multiplex, reverse transcription polymerase chain reaction.
The ArrayPlate qNPA technology offers highly accurate, sensitive and reproducible test results without the need for extraction, reverse transcription or amplification.
These new standards were proposed following publications according to which many of the visual read rapid influenza detection tests had poor sensitivity compared with viral culture and reverse transcription PCR methods.
Given the likely variation in the preparation of RNA, the quality and formulations of commercial reverse transcription (RT)-PCR enzymes and reaction mix components and changes in thermal cyclers since 1988, not surprisingly many, perhaps most, of these assays are not being used in the manner they were originally described.
Real-time reverse transcription (RT)-PCR is the most sensitive method to measure changes in mRNA concentrations.

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