reverse transcriptase polymerase chain reaction


Also found in: Acronyms.

reverse transcriptase polymerase chain reaction (RT-PCR),

a process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.

re·verse trans·crip·tase pol·y·mer·ase chain re·ac·tion

(RT-PCR) (rē-vĕrs' tran-skrip'tās pol-im'ĕr-ās chān rē-ak'shŭn)
A process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.
References in periodicals archive ?
Preoperative combined nested reverse transcriptase polymerase chain reaction for prostate-specific antigen and prostate-specific membrane antigen does not correlate with pathologic stage or biochemical failure in patients with localized prostate cancer undergoing radical prostatectomy.
Sequences were obtained from reverse transcriptase polymerase chain reaction (RT-PCR)--amplified portions of the nucleoprotein (positions 1,157-1,477), based on the reference strain M13215 (17).
Reverse transcriptase polymerase chain reaction (RT-PCR) [3] provides highly sensitive detection of mRNA sequences and has been used widely for the study of gene expression in malignant and physiological states (5, 6).
Reverse transcriptase polymerase chain reaction for prostate specific antigen for the management of prostate cancer [Review].
We did not use direct detection of the virus by reverse transcriptase polymerase chain reaction (RT-PCR).
Enhanced reverse transcriptase polymerase chain reaction for prostate specific antigen as an indicator of true pathologic stage in patients with prostate cancer.
Basal PSA mRNA levels detected by quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR-PSA) in blood from subjects without prostate cancer [Abstract].
Use of a reverse transcriptase polymerase chain reaction (RT-PCR) to detect prostate cells in the blood [Abstract].
Effects of digital rectal examination, cytoscopy, and needle biopsy on the enhanced reverse transcriptase polymerase chain reaction assay [Abstract].
Researchers analyzed 298 prospectively collected, blinded serum samples, using experimental versions of a colorimetric sandwich immunoassay and qualitative reverse transcriptase polymerase chain reaction (RT-PCR) to detect the NMP66 protein complex in the blood of women with breast cancer.
Recent testing of 20 additional samples has been conducted using both immunoassay and reverse transcriptase polymerase chain reaction (RT-PCR) methods.

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