reverse genetics


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reverse genetics

a conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism.
Synonym(s): positional cloning

reverse genetics

methods such as antisense nucleic acids and site-directed mutagenesis that are used to selectively study gene function. Contrasts with classical genetics which depends on the isolation and analysis of cells (animals) with random mutations that can be identified.
References in periodicals archive ?
On the other hand, Reverse genetics is when genotype is known and resulting phenotype is determined (Genotype a Phenotype = G2P).
For bioscience, medical, and veterinary students, researchers, and academics, Bridgen compiles 12 chapters summarizing recent developments in reverse genetics of RNA viruses through applications in each of the core virus groups, including positive sense, negative sense, and double stranded RNA viruses.
We also thank Richard Webby for providing the reverse genetics plasmid pHW2000, Hoffmann-La Roche Ltd.
Reverse genetics (testing whether candidate genes and polymorphisms identified in animal studies as relevant for biological effects also exist in humans and are relevant to the phenotype).
For example, in response to the avian influenza outbreaks throughout the world, FDAH utilized reverse genetics, a cuttingedge technology being used in the development of human vaccines.
Gaithersburg, MD) licensed its proprietary reverse genetics intellectual property to GlaxoSmithKline to support the development and construction of new vaccine strains to produce non-live human influenza vaccines.
has received Food and Drug Administration approval to use reverse genetics technology for its FluMist vaccine production.
Now Yoshihiro Kawaoka and his colleagues at the University of Wisconsin-Madison School of Veterinary Medicine and the University of Tokyo have perfected an advanced method of producing the inactivated "seed" virus used to produce influenza vaccine, a technique known as reverse genetics.
It is a reverse genetics approach for discovering unique variability in plant and animal populations.
Using a method called reverse genetics, Yoshihiro Kawaoka of the University of Wisconsin-Madison and the University of Tokyo and his colleagues engineered a relatively mild flu virus to carry two genes from the 1918 strain.
A Chiron spokesman said: ``There are a number of groups who are working on producing the starting materials, or the seed, for the vaccine using a technique called reverse genetics.