reverse genetics


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reverse genetics

a conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism.
Synonym(s): positional cloning

reverse genetics

methods such as antisense nucleic acids and site-directed mutagenesis that are used to selectively study gene function. Contrasts with classical genetics which depends on the isolation and analysis of cells (animals) with random mutations that can be identified.
References in periodicals archive ?
The first objective will be achieved by (i) focusing on the sequenced, diploid Fragaria vesca, a wild relative of the cultivated strawberry that will serve as a model, (ii) identifying key transcription factors (TFs) regulating fruit ripening by generating a stage- and tissue-specific gene expression map, (iii) using a candidate gene approach and reverse genetics based on gene silencing and TILLING to verify the role of these TFs, and (iv) defining the gene regulatory networks controlling the ripening process via integration of transcriptomic, metabolomic and ChIP-Seq data obtained from the stably silenced and/or TILLED lines.
Now, in a recent study, Yu used reverse genetics technology, which allowed him to generate new vaccines by inserting a gene from the ILTV virus into the NDV LaSota strain.
Since three years ago, we have begun our research on reverse genetics technology, the result of which has been the obtaining of this advanced technology to make various kinds of flu viruses," said Head of the vaccines division at Pars Inc.
But when this method didn't work, the researchers decided to "use this reverse genetics approach," Pruneda-Paz explained.
Scientists first documented the cancer-fighting properties of Newcastle disease virus in the 1950s, but it is only with recent advances in reverse genetics technology that they have turned to the genetically engineered virus as a possible treatment.
Reverse genetics of RNA viruses; applications and perspectives.
By using site-directed mutagenesis and reverse genetics as described (9), we generated recombinant viruses with NA from the pandemic (H1N1) 2009 virus A/Auckland/1/2009 and the remaining genes from A/PR/8/34.
compile 12 articles by researchers in virology, microbiology, infectious diseases, immunology, and related fields in North America, Europe, New Zealand, and Australia who detail current research findings on caliciviruses and related diseases, including norovirus epidemiology, calicivirus contamination of the environment, genome organization and recombination, proteolytic cleavage and viral proteins, viral protein structures, virus-host interactions, reverse genetics and replicon systems, feline calicivirus, murine noroviruses, and lagoviruses.
A technique called reverse genetics was used to create a hybrid between the H1N1 swine flu virus and a tested laboratory strain.
For example, in response to the avian influenza outbreaks throughout the world, FDAH utilized reverse genetics, a cuttingedge technology being used in the development of human vaccines.
Gaithersburg, MD) licensed its proprietary reverse genetics intellectual property to GlaxoSmithKline to support the development and construction of new vaccine strains to produce non-live human influenza vaccines.
Now Yoshihiro Kawaoka and his colleagues at the University of Wisconsin-Madison School of Veterinary Medicine and the University of Tokyo have perfected an advanced method of producing the inactivated "seed" virus used to produce influenza vaccine, a technique known as reverse genetics.