To confirm position of genes into pAHC25, restriction mapping
and bidirectional sequencing of cloned fragment was performed to confirm the construct.
Usefulness of ompl restriction mapping
for avian Chlamydia psittaci isolate differentiation.
In silico restriction mapping
, determination of int22h boundaries, and primer annotations were performed with EditSeq and MapDraw software (LaserGene; DNA Star).
Clones were assembled on the basis of shared gene sequences and on restriction mapping
Moreover, a cladistic analysis using restriction mapping
data grouped the haplotypes from D.
Genomic TaqI digestion coupled with cohybridization with CYP21, TNX, and C4 probes provides direct information about these three genes and alerts the investigator to uncommon configurations that require further analysis by long-range restriction mapping