restriction fragment length polymorphism


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re·stric·tion frag·ment length pol·y·mor·phism (RFLP),

used in genetic analysis of populations or individual relationships. In regions of the human genome not coding for proteins, the distance (in nucleotides on the chromosome) can be different between two genetic markers, usually because of repeated base patterns.

restriction fragment length polymorphism (RFLP)

a difference in the DNA sequences of homologous chromosomes as revealed by different lengths of the restriction fragments produced by enzymatic digestion of a selected region of chromosomes. RFLPs are believed to be inherited according to mendelian laws and have been used to locate the genes associated with several inherited disorders, including Huntington's disease.

re·stric·tion frag·ment length pol·y·mor·phism

(RFLP) (rĕ-strik'shŭn frag'mĕnt length pol'ē-mōrf'izm)
Used in genetic analysis of populations or individual relationships. In regions of the human genome not coding for proteins there is often wide (but measurable) sequence variety between people.

restriction fragment length polymorphism (RFLP)

Variations within a species in the lengths of fragments of DNA caused by RESTRICTION ENZYMES. The variations are caused by mutations that either abolish the normal sites of breakage or create new ones, characteristic of the mutations. RFLP analysis may allow genetic abnormalities to be detected, often before birth, even if the location of the mutated gene or genes is unknown.

restriction fragment length polymorphism (RFLP)

a MUTATION that results in a change in the pattern of restriction fragments generated when a DNA molecule is treated with a RESTRICTION ENZYME (see RESTRICTION ANALYSIS). The RFLP is apparent when the fragments are resolved by AGAROSE GEL ELECTROPHORESIS. RFLPs can be used as reference LOCI for mapping, in relation to specific linked genes (see GENETIC LINKAGE). RFLPs can be used as a diagnostic test for genetic DISEASES, by detecting specific gene defects. The presence of the RFLP may be a direct indication of the gene defect, or may be closely linked to it. Diseases that have heen diagnosed by RFLP analysis include certain types of THALASSAEMIA and HAEMOPHILIA, and by RFLP linkage analysis, HUNTINGTON'S CHOREA.
References in periodicals archive ?
Comparison of subsurface and surface soil bacterial communities in California grassland as assessed by terminal restriction fragment length polymorphisms of PCR-amplified 16S rRNA genes.
However, the GTT haplotype, constructed from the three adjacent restriction fragment length polymorphisms was found to be over-represented among CP cases.
An outbreak of tuberculosis with the human immunodeficiency virus: an analysis using restriction fragment length polymorphism.
flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism (RFLP) analysis are an efficient tool for detection and identification of Leptospira spp.
In 2001, the New York City Bureau of Tuberculosis Control began genotyping isolates for every new TB case with spoligotyping and IS6110-based restriction fragment length polymorphism (RFLP) to improve the efficiency of TB control.
Codon 16/27 genotyping is accomplished with dot-blot oligonucleotide hybridization (16), single-strand conformation polymorphism analysis (9), direct sequencing (17), multiplex PCR (18), denaturation selective amplification and subtractive genotyping (19), allele-specific codon 16 PCR combined with restriction fragment length polymorphism analysis (20), or strand displacement amplification (21).
Restriction fragment length polymorphism, DNA fingerprinting, is a time-consuming, slow, and expensive method of distinguishing members.
For the detection of point mutations (or variant alleles), PCR followed by restriction fragment length polymorphism (RFLP) detection is widely used and is probably one of the simplest methods for detection of known mutations.
By far the commonest method has been PCR amplification followed by restriction fragment length polymorphism (RFLP) analysis.
All the MTB isolates (n = 510; [approximately equal to] 30% of all MTB cases in Madrid), obtained from patients January 2001-December 2002 from 2 tertiary teaching hospitals in Madrid (population 1,383,790), were genotyped by IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping.
The restriction fragment length polymorphism assay was performed in a 15-[micro]L reaction volume containing PCR product and specific restriction enzymes (Earl for the IL-10 polymorphism and Ncol for the TNF-[alpha] polymorphism).
Here we present the characterization of the Bell polymorphism, as well as the application of two simple genotyping assays for its detection: PCR with restriction fragment length polymorphism (RFLP) analysis and allele-specific oligonucleotide (ASO) hybridization.

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