Comparison of subsurface and surface soil bacterial communities in California grassland as assessed by terminal restriction fragment length polymorphisms
of PCR-amplified 16S rRNA genes.
However, the GTT haplotype, constructed from the three adjacent restriction fragment length polymorphisms
was found to be over-represented among CP cases.
An outbreak of tuberculosis with the human immunodeficiency virus: an analysis using restriction fragment length polymorphism
flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism
(RFLP) analysis are an efficient tool for detection and identification of Leptospira spp.
In 2001, the New York City Bureau of Tuberculosis Control began genotyping isolates for every new TB case with spoligotyping and IS6110-based restriction fragment length polymorphism
(RFLP) to improve the efficiency of TB control.
Codon 16/27 genotyping is accomplished with dot-blot oligonucleotide hybridization (16), single-strand conformation polymorphism analysis (9), direct sequencing (17), multiplex PCR (18), denaturation selective amplification and subtractive genotyping (19), allele-specific codon 16 PCR combined with restriction fragment length polymorphism
analysis (20), or strand displacement amplification (21).
Restriction fragment length polymorphism
, DNA fingerprinting, is a time-consuming, slow, and expensive method of distinguishing members.
For the detection of point mutations (or variant alleles), PCR followed by restriction fragment length polymorphism
(RFLP) detection is widely used and is probably one of the simplest methods for detection of known mutations.
By far the commonest method has been PCR amplification followed by restriction fragment length polymorphism
All the MTB isolates (n = 510; [approximately equal to] 30% of all MTB cases in Madrid), obtained from patients January 2001-December 2002 from 2 tertiary teaching hospitals in Madrid (population 1,383,790), were genotyped by IS6110 restriction fragment length polymorphism
(RFLP) and spoligotyping.
The restriction fragment length polymorphism
assay was performed in a 15-[micro]L reaction volume containing PCR product and specific restriction enzymes (Earl for the IL-10 polymorphism and Ncol for the TNF-[alpha] polymorphism).
Here we present the characterization of the Bell polymorphism, as well as the application of two simple genotyping assays for its detection: PCR with restriction fragment length polymorphism
(RFLP) analysis and allele-specific oligonucleotide (ASO) hybridization.