restriction enzyme


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re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).
Synonym(s): restriction enzyme

restriction enzyme

n.
Any of a group of enzymes that catalyze the cleavage of DNA at specific sites to produce discrete fragments, used especially in genetic engineering. Also called restriction endonuclease.

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)
One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

restriction enzyme

One of the many enzymes that break DNA at specific sites. These enzymes are extensively used in research and in GENETIC ENGINEERING. Also known as restriction endonucleases.

restriction enzyme

or

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.
References in periodicals archive ?
Genomic characterization by Restriction enzyme analysis was performed at the Lovelace Respiratory Research Institute.
It can be seen that the positive control has only one band which follows and agrees with the results of Gatphayak et al [3] who stated that the mutation would render the said restriction enzyme inactive due to SNPC119T.
16S rRNA gene PCR product (10 [micro]l of each native diazotrophic bacterial isolates was used to carry out the restriction digestion with four different Tetra-cutter restriction enzymes (TaqI BamHI, HinfI and ae III).
Enzymatic digestion of the volume of 20 ml of buffer and temperature conditions was performed using the restriction enzyme HaeIII .
For cloning in expression vector plasmid pTZ-Map was digested with NdeI and EcoRI restriction enzymes and the liberated gene fragment was ligated in pET-28a.
KC200562 and KC200563) and generated using restriction enzymes during this study, ([dagger]) Predicted fragments of S.
Digestion with HPA 2 restriction enzyme produced 2 bands 209 bp and 99 bp indicating both alleles have G polymorphs which are true with sample number SD2 56 of which undigested and digested products are shown in lane 4 and 5 respectively.
monocytogenes strains was determined by PFGE of DNA digested with the restriction enzymes ApaI and SmaI, and electrophoresed on a Chef DRII system (Bio-Rad Laboratories, Hercules, CA).
The presence of the LPG3 gene in pLEXSY-hyg2 was confirmed by BglII and NheI restriction enzymes and PCR amplification.
2], [epsilon]) which pops the symbol from the stack acts by using also the restriction enzyme Bg/I (Appendix 3).
Examination of the reacted DNA with single restriction enzyme was observed by 2% agarose gel electrophoresis and indicated the complete cleavage, as seen by disappearance of the genomic DNA band and uniform smear of cleaved DNA (Chapaval et al.
The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining.

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