restriction enzyme


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re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).
Synonym(s): restriction enzyme

restriction enzyme

n.
Any of a group of enzymes that catalyze the cleavage of DNA at specific sites to produce discrete fragments, used especially in genetic engineering. Also called restriction endonuclease.

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)
One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

restriction enzyme

One of the many enzymes that break DNA at specific sites. These enzymes are extensively used in research and in GENETIC ENGINEERING. Also known as restriction endonucleases.

restriction enzyme

or

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.
References in periodicals archive ?
2007) used various restriction enzymes like AluI, BsaJI, and Rsal to identify pork from different mixtures of meat; chicken, pig and cattle in various modifications.
KC200562 and KC200563) and generated using restriction enzymes during this study, ([dagger]) Predicted fragments of S.
A new idea is to use a restriction enzyme which has two separate recognition sites.
We used gel electrophoresis and densitometry analysis of restriction enzyme digests to obtain an estimate of the relative methylation of relevant CpGs (Fig.
Students will realize that although the two DNA molecules are the same size to begin with, when the molecules are cut, fragments of various sizes are produced depending on the location of the restriction enzyme site.
At this stage, the plasmid DNA was digestable by most restriction enzymes for a quick DNA analysis.
For TRFLP analysis, PCR amplicons are fluorescently tagged at the 5' end and cut with restriction enzymes, and the terminal restriction fragments can then be quantified by electrophoresis to yield an index of microbial composition, relative abundance, and overall diversity (Blackwood et al.
30) Smith, H O and K W Wilcox, 'A restriction enzyme from Hemophilus influenzae', Journal of Molecular Biology, vol.
The use of restriction enzymes to cleave the DNA can reveal variation in the mtDNA sequence at specific sites, and individuals or species can often be distinguished by the presence or absence of these sites.
A restriction enzyme makes two incisions, one through each phosphate backbone of the double helix DNA molecule.
Techniques mastered during the program include agarose, polyacrylamide and protein gel electrophoresis, DNA sequencing, gene mapping, restriction enzyme cleavage of DNA, recombinant gene technology, cloning, polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (rtPCR), Southern and Western blotting, analysis of restriction fragment polymorhisms (RFLP), analysis of variable number tandem repeats (VNTR's), DNA fingerprinting for paternity and forensics analysis, protein analysis and bioprocessing.

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