restriction endonuclease


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re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).
Synonym(s): restriction enzyme

restriction endonuclease

[ristrik′shən en′dōno̅o̅′klē·ās]
Etymology: L, restringere + Gk, endon, within; L, nucleus, nut kernel; Fr, diastase, enzyme
an enzyme that cleaves DNA at a specific site. Each of the many endonucleases isolated from various bacteria acts at a different site, making it possible for researchers to divide DNA molecules at selected locations. See also restriction fragment length polymorphism.

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)
One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

restriction enzyme

or

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.

restriction endonuclease

one of over 200 enzymes isolated from bacteria that cleave any DNA molecule at specific sites which are usually palindromes of 4 to 10 or so nucleotides to yield a collection of restriction DNA fragments that can be separated, usually by electrophoresis in agarose or polyacrylamide gels, and which are highly characteristic for a particular DNA. They evolved as a defense mechanism for bacteria in that they modify the bacteria's own DNA by methylation which blocks the restriction fragmentation function but allows restriction of any foreign DNA that enters the cell. Called also restriction-modification enzyme. See also restriction map.
References in periodicals archive ?
There are three classes or types of restriction endonucleases, (10) but it is the type II restriction endonucleases that are the basis of recombinant DNA technology and the 'scissors' of modern molecular methods.
hongkongensis based on the two restriction endonucleases in our key, but several other endonucleases will differentiate between the two (JFC, unpublished data).
1) were analyzed to survey broadly the species' mtDNA variability using 12 restriction endonucleases (Appendix 1).
Hae III restriction endonuclease analysis of PCR product revealed three different banding patterns consisting of 2-3 DNA fragments ranging from 0.
Amplification is performed using primers that have a target-specific 3' region and a 5'-oligonucleotide tail containing a fluorophore-quencher pair separated by nucleotides carrying the recognition sequence of an exceptionally thennostable restriction endonuclease, PspGI (28).
edule banded with the restriction endonuclease HaeIII were acquired with a CCD camera (Axiocam, ZEISS) coupled to a ZEISS Axioplan 2 Imaging microscope.
PCR amplification and restriction endonuclease analysis of a 65-kiloDalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria [ERRATUM 1995;33:1686].
Enzyme digestion was performed by HpaII, a methylation-sensitive restriction endonuclease that recognized and cut the DNA sequence CCGG and whose action would be blocked by CpG methylation.
After PCR amplication, resulting amplicons (10 [micro]L) were digested to completion with the restriction endonuclease Dde I (10U) (cleavage site sequence CTGCA[down arrow]G; Toboyo Co.
We transferred 2 [micro]L of the PCR product to a tube containing 2 units of restriction endonuclease ItaI in 1 X restriction buffer H (both from Roche Molecular Biochemicals); the total assay volume was 10 [micro]L.
Restriction endonuclease analysis of the RV1 genome indicated that the genome was [approximately equal to] 20-30 kbp (data not shown).
The target DNA is single stranded and is generated from genomic DNA via an asyininetric PCR reaction, after which the target amplifier and anchor are hybridized to form a 3-way DNA junction that contains the recognition site for a thermostable restriction endonuclease (Fig.

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