restriction endonuclease

endonuclease /en·do·nu·cle·ase/ (-noo´kle-ās) any nuclease specifically catalyzing the hydrolysis of interior bonds of ribonucleotide or deoxyribonucleotide chains.

restriction endonuclease an endonuclease that hydrolyzes DNA, cleaving it at an individual site of a specific base pattern.

[ristrik′shən en′dōno̅o̅′klē·ās]

Etymology: L, restringere + Gk, endon, within; L, nucleus, nut kernel; Fr, diastase, enzyme

an enzyme that cleaves DNA at a specific site. Each of the many endonucleases isolated from various bacteria acts at a different site, making it possible for researchers to divide DNA molecules at selected locations. See also restriction fragment length polymorphism.

restriction endonuclease

one of over 200 enzymes isolated from bacteria that cleave any DNA molecule at specific sites which are usually palindromes of 4 to 10 or so nucleotides to yield a collection of restriction DNA fragments that can be separated, usually by electrophoresis in agarose or polyacrylamide gels, and which are highly characteristic for a particular DNA. They evolved as a defense mechanism for bacteria in that they modify the bacteria's own DNA by methylation which blocks the restriction fragmentation function but allows restriction of any foreign DNA that enters the cell. Called also restriction-modification enzyme. See also restriction map.

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activating enzyme allosteric site binding site caprine Cloning Vector cohesive end DNA Footprinting EcoR1 endonuclease enzyme enzyme induction methylases molecular epidemiology Palindrome recombinant recombinant DNA technology repressible enzyme restriction enzyme restriction fragment length polymorphism (RFLP)	coli protein expression vector using polymerase chain reaction (PCR), restriction endonuclease digestion, and DNA ligation. Structural studies of human mitochondrial aconitase by Journal of the Alabama Academy of Science Rotavirus subgroup characterisation by restriction endonuclease digestion of a cDNA fragment of the VP6 gene. Novel human rotavirus genotype G5P[7] from child with diarrhea, ... by Esona, Mathew D.; Geyer, Annelise; Banyai, Krisztian; Page, Nicola; Aminu, Maryam; Armah, George E.; / Emerging Infectious Diseases 2008) greatly facilitated optimization of the key by allowing us to perform virtual restriction endonuclease digestion (VRED) analyses, which saved time by eliminating much of the laboratory-intense trial-and-error usually associated with identifying informative restriction endonucleases. Discrimination of nine Crassostrea oyster species based upon ... by Cordes, Jan F.; Xiao, Jie; Reece, Kimberly S. / Journal of Shellfish Research	restis restitution restless Restless Leg Syndrome restless legs syndrome restlessness Restocalm Reston virus restoration restoration contour restoration of cusps restorative restorative dentistry RESTORE Restoril restrainer Restraining Garment restraint Restraint Asphyxia restraint in bed restraint of trade restricted area restricted substances restriction restriction digest restriction endonuclease restriction enzyme restriction fragment restriction fragment length polymorphism restriction fragment length polymorphism (RFLP) restriction map restriction mapping restriction site restriction-site polymorphism restrictive barrier restrictive cardiomyopathy restrictive covenant restrictive disease restrictive lung disease restructuring Restylane resume resurrection plant resuscitate resuscitation resuscitation 1 resuscitation 2 resuscitation: fetus resuscitation: neonate resuscitator Resveratrol	restrictedly restrictedly restrictedly restrictedly restrictedness restrictedness restrictedness restrictedness restricter restricter restricting restricting Restricting Antiabortion Protests Restricting Antiabortion Protests Restricting Antiabortion Protests Restricting Antiabortion Protests restriction restriction restriction restriction restriction restriction Restriction and Modification Restriction digest restriction e restriction e restriction e restriction e's restriction e's restriction e's restriction endonuclease restriction endonuclease analysis restriction endonuclease analysis Restriction Endonuclease Digestion Profile Restriction Endonuclease Fragment Length Variations Restriction Endonuclease Fragmentation Pattern Restriction Endonuclease In Situ Digestion Restriction endonucleases Restriction endonucleases Restriction endonucleases restriction enzyme restriction enzyme restriction enzyme Restriction Enzyme Analysis Restriction Enzyme Fragmentation Pattern Restriction enzyme, endonuclease Restriction enzyme, endonuclease Restriction enzyme, endonuclease Restriction Enzyme-Mediated Integration Restriction enzymes Restriction enzymes Restriction enzymes Restriction Factor 1 restriction fragment restriction fragment restriction fragment Restriction Fragment Differential Display Restriction Fragment End Labeling Restriction Fragment Length Difference Restriction Fragment Length Dimorphism Restriction Fragment Length Pattern

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References in periodicals archive

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activating enzyme
allosteric site
binding site
caprine
Cloning Vector
cohesive end
DNA Footprinting
EcoR1
endonuclease
enzyme
enzyme induction
methylases
molecular epidemiology
Palindrome
recombinant
recombinant DNA technology
repressible enzyme
restriction enzyme
restriction fragment length polymorphism (RFLP)

coli protein expression vector using polymerase chain reaction (PCR), restriction endonuclease digestion, and DNA ligation.

Structural studies of human mitochondrial aconitase by Journal of the Alabama Academy of Science

Rotavirus subgroup characterisation by restriction endonuclease digestion of a cDNA fragment of the VP6 gene.

Novel human rotavirus genotype G5P[7] from child with diarrhea, ... by Esona, Mathew D.; Geyer, Annelise; Banyai, Krisztian; Page, Nicola; Aminu, Maryam; Armah, George E.; / Emerging Infectious Diseases

2008) greatly facilitated optimization of the key by allowing us to perform virtual restriction endonuclease digestion (VRED) analyses, which saved time by eliminating much of the laboratory-intense trial-and-error usually associated with identifying informative restriction endonucleases.

Discrimination of nine Crassostrea oyster species based upon ... by Cordes, Jan F.; Xiao, Jie; Reece, Kimberly S. / Journal of Shellfish Research

restis

restitution

restless

Restless Leg Syndrome

restless legs syndrome