restriction endonuclease


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re·stric·tion en·do·nu·cle·ase

one of many endonucleases isolated from bacteria that cleave or hydrolyze (cut) foreign double-stranded DNA chains at specific recognition sites defined by DNA sequences; these endonucleases have become standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences and are sometimes referred to as a "chemical knife," usually named by a three- or four-letter abbreviation of the name of the organism from which isolated (for example, EcoB from Escherichia coli, strain B).
Synonym(s): restriction enzyme

restriction endonuclease

[ristrik′shən en′dōno̅o̅′klē·ās]
Etymology: L, restringere + Gk, endon, within; L, nucleus, nut kernel; Fr, diastase, enzyme
an enzyme that cleaves DNA at a specific site. Each of the many endonucleases isolated from various bacteria acts at a different site, making it possible for researchers to divide DNA molecules at selected locations. See also restriction fragment length polymorphism.

re·stric·tion en·do·nu·cle·ase

(rĕ-strik'shŭn en'dō-nū'klē-ās)
One of many endonucleases isolated from bacteria that hydrolyze (cut) double-stranded DNA chains at specific sequences, thus inactivating a foreign (viral or other) DNA and restricting its activity; standard laboratory devices for making specific cuts in DNA as a first step in deducing sequences.
Synonym(s): restriction enzyme.

restriction enzyme

or

restriction endonuclease

an endonuclease that recognizes a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site) and cleaves both strands of DNA at or near that site. The enzyme cuts the DNA, generating restriction fragments with OVERHANGING ENDS or BLUNT ENDS. See also COHESIVE ENDS.

restriction endonuclease

one of over 200 enzymes isolated from bacteria that cleave any DNA molecule at specific sites which are usually palindromes of 4 to 10 or so nucleotides to yield a collection of restriction DNA fragments that can be separated, usually by electrophoresis in agarose or polyacrylamide gels, and which are highly characteristic for a particular DNA. They evolved as a defense mechanism for bacteria in that they modify the bacteria's own DNA by methylation which blocks the restriction fragmentation function but allows restriction of any foreign DNA that enters the cell. Called also restriction-modification enzyme. See also restriction map.
References in periodicals archive ?
There are three classes or types of restriction endonucleases, (10) but it is the type II restriction endonucleases that are the basis of recombinant DNA technology and the 'scissors' of modern molecular methods.
It is likely that many of the 12 restriction endonucleases utilized did not yield differing haplotypes because these areas of the amplicon are highly conserved.
Systematic relationships among waterfowl (Anatidae) inferred from restriction endonuclease analysis of mitochondrial DNA.
patent 8,921,332 issued on December 30th, 2014, which claims the use of chimeric restriction endonucleases for directing chromosomal gene editing in cells by homologous recombination.
To construct the physical map of the chromosome of Psm, three rare-cutting restriction endonucleases were chosen based on G+C content of P syringae strains, several enzymes with A+T rich recognition sequences were tested, among these enzymes PacI (TTAATTAA), PmeI (GTTTAAAC) and SwaI (ATTTAAAT) were selected which originated 14, 15 and 16 fragments, respectively.
Results: Recombinant plasmid pcDNA3--HBX was confirmed by restriction endonucleases digestion and colony-PCR.
The amplified fliC gene was cleaved with HhaI restriction endonuclease (Invitrogen), when fliC(M) primers were used, and RsaI restriction endonuclease (Invitrogen), when fliC(F) primers were used.
The polymorphism at nucleotide 1843 of the hal gene has been characterized by PCR amplification associated to restriction endonuclease assay (PCRREA) using blood or muscle biopsy as the source of genomic DNA (HOUDE et al.
Restriction endonuclease analysis (REA): 10 [micro]g of genomic DNA was digested with 100 units each of Hind III, Hae III and EcoRI restriction enzymes (Boehringer Mannheim, Germany) according to the manufacturer's instruction.

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