restriction digest


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restriction digest

a unique family of DNA fragments produced by digestion of a given DNA molecule by a particular restriction enzyme; usually visualized by agarose gel electrophoresis and ethidium bromide or other fluorescent procedures.
References in periodicals archive ?
To investigate the fragment length-dependent sequencing bias, we prepared a restriction digest of [lambda] DNA (Invitrogen).
Our PCR, restriction digest, and electrophoresis protocols were adapted from Gruntzig et al.
Four distinct phylogenetic lineages of human serotype III GBS have been identified by PFGE of restriction digest patterns and designated restriction digest patterns type III-1, III-2, III-3, and III-4 (11).
To quantify the proportion of the DNA that could be digested, we divided the density of the full-length PCR product after the restriction digest by the density before the restriction digest.
The Agilent 2100 bioanalyzer is now offered with five LabChip kits including three for the high-resolution analysis of DNA samples, such as PCR products and restriction digest fragments, one for the analysis of RNA samples typically used in gene expression applications, and the new protein assay.
pseudomallei were typed by PFGE of an XbaI restriction digest of bacterial chromosomal DNA (Figure).
An aliquot of the restriction digest is taken for PFGE analysis as a control for DNA preparation; the remaining prepared DNA molecules are analyzed with the DLA system.
These instruments are being currently used for a wide variety of applications including microsatellite analysis, restriction digest sample quality checks as well as oligonucleotide quality checks.
Each sample was run in triplicate using one lane for the restriction digest and an adjacent control lane for the uncut DNA.
Restriction digest products were size fractionated by electrophoresis on a 2% agarose gel with 1 mg/L ethidium bromide for 20 min at 200 V and detected directly under ultraviolet light.
One microliter of undiluted restriction digest was amplified in the second PCR in a 20-[micro]L volume in 1 x PCR buffer containing 1.
Evaluation of 100 human DNA samples for factor V Leiden by both the restriction digest and the fluorescence protocols yielded identical genotyping results by both methods: 60 samples were wild-type, 37 were heterozygous, and 3 were homozygous for the factor V Leiden mutation.