restriction analysisthe analysis of DNA molecules by the use of RESTRICTION ENZYMES. The DNA is cleaved with the enzyme(s) and the fragments obtained are generally separated by GEL ELECTROPHORESIS. The pattern of restriction fragments produced can be used for various purposes:
- (a) to detect fragments of a specific gene using a nucleic acid PROBE;
- (b) to detect the presence of mutations (see RESTRICTION FRAGMENT LENGTH POLYMORPHISM);
- (c) to compare DNA from different individuals (see FINGERPRINTING);
- (d) to construct physical maps, called restriction maps, of DNA molecules, that indicate the position of restriction sites in the molecules.