restriction analysis

restriction analysis

the analysis of DNA molecules by the use of RESTRICTION ENZYMES. The DNA is cleaved with the enzyme(s) and the fragments obtained are generally separated by GEL ELECTROPHORESIS. The pattern of restriction fragments produced can be used for various purposes:
  1. (a) to detect fragments of a specific gene using a nucleic acid PROBE;
  2. (b) to detect the presence of mutations (see RESTRICTION FRAGMENT LENGTH POLYMORPHISM);
  3. (c) to compare DNA from different individuals (see FINGERPRINTING);
  4. (d) to construct physical maps, called restriction maps, of DNA molecules, that indicate the position of restriction sites in the molecules.
References in periodicals archive ?
White colonies were further grown in LB broth medium with ampicillin, plasmid was isolated and subjected to restriction analysis using EcoRI and SalI.
Amplified Ribosomal DNA Restriction Analysis (ARDRA) is the extension of the RFLP (restriction fragment length polymorphism) technique encoding the small (16s) ribosomal subunit of bacteria.
2013) and restriction analysis of chloroplast fragments (Parfitt and Badenes, 1997) in order to resolve the phylogenetic relationships.
The objectives of this study were to investigate the stem and root nodulation associated with these species and to perform phenotypic and molecular characterization, in the latter case by restriction analysis of the 16S rDNA gene, of bacterial isolates obtained from nodules of species of the Discolobium and Aeschynomene genera in the Pantanal.
The isolates from lungs, kidney, heart, intestine, liver, and bone marrow all harbored the same virulence-associated factors (iucD, colV, iss, mat, fimC, ompA, traT crl, csgA vgrG, and hcp), yielded the same band pattern in amplified ribosomal DNA restriction analysis, and were allocated to the E coli Reference Collection group B1.
The restriction analysis by PstI of the positive PCR samples further subcategorized into two genotypes (Fig.
Amplified fragments were confirmed by restriction analysis and DNA sequencing.
we compared nucleotide sequence identity to reference strains and carried out multilocus analysis using ompA-ompB sequences and in silico PstI and RsaI restriction analysis of ompA sequences (7).
Detection of Recombinants: To analyze the presence and orientation of the DNA insert into recombinant clones, some methods including colony PCR, restriction analysis and sequencing were performed depending on the purpose.
We will use hMeDIP-sequencing and combined glycosylation restriction analysis to measure the differential levels of 5-methylcytosine and 5-hydroxymethylcytosine marks.