Clontech's initial product offering includes Guide-it kits for in vitro production and screening of single guide RNAs (sgRNAs) and a method to confirm the presence of targeted mutations using direct PCR and Clontech's Guide-it Resolvase
ISTN3X; 96% identical), comprising one of Tn3-like inverted repeats and putative coding regions for transposase, resolvase
(also called repressor), and ampicillin resistance.
All three patents covering resolvase
technologies are assigned to Variagenics.
Heteroduplexes are cleaved by the resolvase T4 endonuclease VII, usually in both strands within 3-6 by 3' from the mismatch (21).
X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture.
EMD uses a resolvase enzyme cloned from bacteriophage [T.
The double-stranded DNA fragments resulting from resolvase cleavage can be detected by conventional analytical methods such as gel electrophoresis.
After incubation with resolvases or chemicals, the products are resolved electrophoretically side by side to score for the presence of mismatch-cleaved molecules (9-11).
Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvases.
Variance Imaging(TM) is based on Enzyme Mutation Detection(TM) (EMD(TM)) technologies which enable the use of enzymes termed resolvases to cleave double-stranded DNA at any site where a mismatch between DNA strands produces an abnormal DNA structure.
Richard Cotton and Rima Youil of the Mutation Research Center in Melbourne Australia relating to the use of resolvases for mismatch recognition.
So far, bacteriophage resolvases
have been applied to the analysis of heterozygous states.