2]- antiplasmin vWD N [up arrow] Inherited platelet N [down arrow] defects Inherited bleeding Plasma condition Other tests half-life [down arrow] Fibrinogen Platelet aggregation 2--4 d N/[down arrow] * Dysfibrinogenaemia FDPs may be [up arrow] -- (bleeding disorder) Reptilase
time [up arrow] [down arrow] Prothrombin 2-stage assay abnormal 3--4 d [down arrow] Factor V Factor V assay 36 h [down arrow] Factor VII Factor VII assay 4-6 h [down arrow] Factor VIII Factor VIII assay 10-14 h [down arrow] Factor IX Factor IX assay 25 h [down arrow] Factor X Factor X assay 40-60 h [down arrow] Factor XI Factor XI assay 50 h [down arrow] Factor XIII Abnormal UCLT, 9-12 d [down arrow] fibrinogen [down arrow] Abnormal UCLT, [down 72 h [[alpha].
In the ActF channel, reptilase
and factor XIII are used to produce a clot with no platelet contribution.
Snake venom thrombin-like enzymes: from reptilase
Degradation of fibrin by reptilase
and thrombin fibrinogen and its derivatives.
This band corresponded to the presence of residual fibrinogen (1), because it disappeared after reptilase
A normal reptilase
time despite PTT >180 seconds (Table 1) eliminated dysfibrinogenemia as the cause of the coagulopathy and indicated the presence of heparin.
This is achieved by adding reptilase
(batroxobin), which generates fibrin without platelet activation, and factor [XIII.
Currently, several thrombolytic agents such as streptokinase, urokinase, prourokinase, reteplase (r-PA), alteplase (t-PA), reptilase
, brinase and anisoylated purified streptokinase activator complex (APSAC) are available for clinical use (1).
We then performed PT; activated partial thromboplastin time (APTT); fibrinogen (clottable and immunological); d-dimer; reptilase
time; thrombin times; coagulation factors II, V, VII, VIII, IX, X, XI, XII; anti-thrombin (AT); alpha-2 antiplasmin (AP); protein C (PC); mixing studies (using rabbit normal plasma); lupus anticoagulant screening and confirmatory testing (dilute Russell's viper venom [American Diagnostica, Greenwich CT], STACLOT LA, [Diagnostica-Stago, Inc.
A 49-year-old African-American female with history of hepatitis C and hypertension was investigated for a possible dysfibrinogenemia when an abnormal thrombin time (TT) and reptilase
time (RT) were reported by the laboratory.
An alternative screening test is the reptilase
Serum protein electrophoresis: reptilase
treatment is superior to ethanol precipitation for specific removal of fibrinogen from heparinized plasma samples.