Through the application of its Hybrid mRNA Delivery System(TM), PhaseRx has demonstrated high-level expression of the luciferase reporter protein
specifically in liver hepatocytes in vivo following the intravenous administration of luciferase mRNA.
3C shows as the simultaneous presence of sub-inhibitory concentration of levofloxacin and increasing concentrations of curcumin have no influence on the relative activity of the reporter protein
The only reported study using a fluorescent reporter protein
(enhanced green fluorescent protein (EGFP)) in a haliotid species was during the transfection of ova, embryos, and sperm in Haliotis discus hannai by Wang et al.
The signaling capacities of both AP and acridinium were found to be damaged by addition of sulfhydryl groups; therefore, conjugates using these materials were prepared with maleimide-modified reporter protein
alternating with sulfhydryl-modified BSA.
NanoLuc Luciferase possesses a host of physical properties that make it an excellent reporter protein
To study the promoter and enhancer control of gene expression, firefly luciferase is widely used as a reporter protein
because it has high sensitivity and a broad linear range.
With PCA, the gene encoding a reporter protein
is rationally dissected into two or more fragments.
SwitchGear has created an optimized luminescent reporter RenSP by increasing the gene's overall light output and decreasing the half-life of the reporter protein
," said Nathan Trinklein, Ph.
Two of the allowed patent applications disclose non-invasive methods for detecting gene expression within a living mammal, where that mammal includes a gene encoding either a bioluminescent or fluorescent reporter protein
linked to a selected promoter that drives its expression.
Contract Notice: NHS Scotland Blood Transfusion Service has a requirement for one 4 Laser Flow Cytometer for the detailed analysis of donor or patient platelets, erythrocytes and leucocytes (including rare cells such as stem cells and antigen-specific T cells) and for more specialist applications including: the co-detection of reporter proteins
and multiple surface markers or the analysis of cytokines; by the Scottish National Blood Transfusion Service (SNBTS) Research, Development & Innovation (RDI) Directorate.
Many proteins are used as reporter proteins
, for example chloramphenicol acetyltransferase (CAT) , [beta] galactosidase and luciferase.
Greater use of label-free detection systems will also allow the use of unmodified cells, removing the need to insert reporter proteins
that can affect cell function.