recombinant DNA technology

recombinant

1. the new cell or individual that derives some of its genetic material from one parent and some from another, genetically different parent.

2. pertaining or relating to such cells or individuals.

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recombinant DNA technology

a mixture of technologies developed in the 1970s that include (a) specific cleavage of DNA by restriction endonucleases; (b) nucleic acid hybridization which makes it possible to identify specific sequences of DNA or RNA; (c) DNA cloning whereby a specific DNA fragment is integrated (spliced) into a rapidly replicating, high yielding genetic element (plasmid or virus) so that it can be amplified in bacteria or yeast; (d) DNA sequencing of the nucleotides in a cloned DNA fragment.

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recombinant DNA technology

Molecular biology The chopping of DNA and moving the pieces, permitting direct examination of the human genome, and identifying the genetic components of various disorders; RDT is also used to develop diagnostic tests, drugs and biologicals for treating disease; the constellation of techniques that comprise 'genetic engineering', in which a gene producing a protein of interest from one organism is spliced into the genome of another organism–eg, a phage DNA integrated into a plasmid is inserted into a 'carrier' bacterium. See Genetic engineering, pBR322, PCR.