reannealing


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reannealing

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In reannealing step, nascent strand on the template strand can be slipped either in forward direction or in backward direction, resulting in "bulge" formation.
After reannealing, products were treated with SURVEYOR nuclease and SURVEYOR enhancer S (Transgenomics, Omaha, NE, USA) following the manufacturer's recommended protocol.
That is, after denaturation and reannealing of nascent strand to template, the polymerase may be "ahead of "or "behind" where it was when it left.
The amplification with equimolar concentrations of primer entails the reannealing of amplified strands [hook effect (49)] and circumvents the efficient hybridization of the universal [Q.
Although the assay for measurement of strand breaks employed yields a result that is the net effect of induction of breaks and their reannealing, the increase in DNA breaks due to incision at mitomycin C damaged sites (Table 2) indicates that morphine does not block incision but interferes with a subsequent step in excision repair.
Although this is commonly referred to as an extension step, a major purpose is to allow reannealing of the PCR product into double-stranded DNA so it can be used for cloning or visualized using ethidium bromide after gel electrophoresis.
Although this is commonly referred to as an extension step, a major purpose is to allow reannealing of the PCR product into double-stranded DNA, so it can be used for cloning or visualized using ethidium bromide after gel electrophoresis.
The reannealing temperature varied for each primer (Table I).
gigas, although the presence of extra heteroduplex bands (apparently resulting from reannealing of single strands from different species in the PCR process) added a layer of complexity to the key (Camara et al.
For 2004 samples, reannealing rate assays, described below, were run in a 96-well-plate format on a quantitative PCR machine (ABI 7700); 2005 samples were run in a 384-well-plate format on the similar ABI 7900.
Such tools can be salvaged by reannealing and rehardening, but with some risk of surface-condition problems and size change.