reannealing


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reannealing

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That is, after denaturation and reannealing of nascent strand to template, the polymerase may be "ahead of "or "behind" where it was when it left.
The amplification with equimolar concentrations of primer entails the reannealing of amplified strands [hook effect (49)] and circumvents the efficient hybridization of the universal [Q.
Although the assay for measurement of strand breaks employed yields a result that is the net effect of induction of breaks and their reannealing, the increase in DNA breaks due to incision at mitomycin C damaged sites (Table 2) indicates that morphine does not block incision but interferes with a subsequent step in excision repair.
Although this is commonly referred to as an extension step, a major purpose is to allow reannealing of the PCR product into double-stranded DNA so it can be used for cloning or visualized using ethidium bromide after gel electrophoresis.
The reannealing temperature varied for each primer (Table I).
gigas, although the presence of extra heteroduplex bands (apparently resulting from reannealing of single strands from different species in the PCR process) added a layer of complexity to the key (Camara et al.
Such tools can be salvaged by reannealing and rehardening, but with some risk of surface-condition problems and size change.
These extra bands are heteroduplex DNA molecules formed after denaturation and reannealing of species-specific PCR products (i.
Before loading onto the column, we denatured the PCR product by heating to 94[degrees]C for 5 min and reannealing slowly by decreasing the temperature 1[degrees]C/min until the temperature reached 25[degrees]C (70 min total).
This approach combines the analytical strategy and statistics of nucleic acid reannealing kinetics (Marsh & Fielman 2005) with isothermal protein denaturation kinetics (Epps et al.
To identify the homozygotes for these polymorphisms, aliquots of a known wild-type sample were pooled with the unknown DNA samples before the reannealing step to enable heteroduplex formation before injection into denaturing HPLC.
20) have described multiple-band artifacts that are produced by improper reannealing of repetitive microsatellite sequences when too many PCR cycles are used.