primer(redirected from random primer method)
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1. A molecule, which may be a small polymer, that initiates the synthesis of a larger structure. Synonym(s): starter
2. A pheromone that causes a long-term physiologic change.
Genetics A segment of DNA or RNA that is complementary to a given DNA sequence and that is needed to initiate replication by DNA polymerase.
1 a short piece of DNA or RNA complementary to a given DNA sequence that acts as a point at which replication can proceed, as in a polymerase chain reaction.
2 a molecule, such as a small polymer, that induces the synthesis of a larger molecule.
1. A short RNA sequence paired with one DNA strand that provides a free 3’-OH end on which synthesis of a deoxyribonycleotide chain can start.
2. The short nucleotide sequence used to start the POLYMERASE CHAIN REACTION (PCR) process.
primera short OLIGONUCLEOTIDE, which is required for the functioning of various POLYMERASES. In DNA REPLICATION, the primer is a short RNA sequence that anneals to the DNA TEMPLATE and is extended by DNA POLYMERASE. In the POLYMERASE CHAIN REACTION, the primer is a short oligonucleotide that initiates the PCR. In OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS, the synthetic oligonucleotide used to introduce the mutation is a mutagenic primer that initiates DNA synthesis.
oligonucleotide which is hydrogen-bonded to the template strand of DNA; required for the replication of DNA by polymerase.
random primer method
a method of labeling DNA in vitro.
an orderly method for sequencing DNA which involves obtaining some sequence, then making an oligonucleotide primer to the end of the sequence obtained and using it as a primer for the next sequencing reaction and repeating this until the whole sequence is determined.