assay buffer (Beyotime, Shanghai, China) was used to lyse cells for 10 min on ice.
The muscle samples were powdered with liquid nitrogen and lysed using a combination of a protease inhibitor (Sigma Aldrich Company, USA; S8820) and a radioimmunoprecipitation
assay (RIPA) buffer (Sigma Aldrich Company, USA; R0278).
Total protein was extracted with 1x radioimmunoprecipitation
lysis buffer and separated on a 10% polyacrylamide gel followed by transfer to a nitrocellulose membrane (Bio-Rad, USA).
Cells were lysed using radioimmunoprecipitation
assay (RIPA) buffer (50 mM Tris-HCl, pH 8.
Raritan, NJ, USA), followed by a research radioimmunoprecipitation
assay (RIPA) for supplemental testing of all repeat reactive donations.
oil red O staining; PBS, phosphate buffered saline; PPAR[gamma], peroxisome proliferator-activated receptor [gamma]; PTP1B, protein-tyrosine phosphatase; RIPA buffer, radioimmunoprecipitation
assay buffer; SDS, sodium dodecyl sulphate; SREBP, sterol regulatory element-binding protein: TAG, triglycerides.
Urethral tissue was collected and samples were prepared by homogenizing in radioimmunoprecipitation
assay lysis buffer (Santa Cruz Biotechnology, Santa Cruz, CA, U.
Cell lysates from cultured human adipose tissue were washed in ice-cold PBS followed by homogenization with radioimmunoprecipitation
assay lysis buffer (Upstate) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) at 4[degrees]C for 30 min.
Samples of renal cortex tissue and HK-2 cells were lysed in radioimmunoprecipitation
UMSCC1 transductant cells were lysed in radioimmunoprecipitation
assay buffer containing 1X protease inhibitors (Cat.
In 85-90% of cases, the diagnosis is made with an acetylcholine receptor antibody (AChR) radioimmunoprecipitation
5 1L protease inhibitor cocktail, and 1455 1L radioimmunoprecipitation
assay buffer (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA).