radioimmunoassay


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Related to radioimmunoassay: ELISA

radioimmunoassay

 (RIA) [ra″de-o-im″u-no-as´a]
a sensitive assay method that can be used for the measurement of minute quantities of specific antibodies or any antigen, such as a hormone or drug, against which specific antibodies can be raised. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit, and hormone that has been labeled with a radioisotope. These are mixed with the assay specimen and the antigen (hormone) bound to antibody is separated from the unbound antigen by chromatography or other means. Because any hormone in the assay specimen competes with the radiolabeled hormone for antibody binding sites, the amount of hormone in the specimen is inversely proportional to the radioactivity of the bound fraction or directly proportional to the activity of the free fraction. This is the standard method for clinical laboratory measurements of hormones and is also used for therapeutic drug monitoring, drug abuse screening, and other laboratory tests.

ra·di·o·im·mu·no·as·say (RIA),

(rā'dē-ō-im'yū-nō-as'sā),
An immunologic (immunochemical) procedure that uses competitive inhibition between a radioisotope-labeled antigen or other substance and unlabeled antigen for antiserums, binding as a means to quantify the agent of interest; any method for detecting or quantitating antigens or antibodies using radiolabeled reactants. Minute quantities of enzymes, hormones, or other substances can be assayed.

radioimmunoassay

/ra·dio·im·mu·no·as·say/ (-im″u-no-as´a) a highly sensitive and specific assay method that uses the competition between radiolabeled and unlabeled substances in an antigen-antibody reaction to determine the concentration of the unlabeled substance, which may be an antibody or a substance against which specific antibodies can be produced.

radioimmunoassay

(rā′dē-ō-ĭm′yə-nō-ăs′ā, -ĭm-yo͞o′-)
n. Abbr. RIA
A technique used to quantitate minute amounts of a substance, such as a hormone, in blood or another liquid. It involves the use of radioisotopes and of antibodies to the substance being investigated.

radioimmunoassay (RIA)

[rā′dē·ō·im′yənō·as′ā]
Etymology: L, radius + immunis, free; Fr, essayer, to try
a technique in radiology used to determine the concentration of an antigen, antibody, or other protein in the serum. The technique involves the injection of a known amount of a radioactively labeled substance that reacts with the protein in question.

radioimmunoassay

see RIA.

ra·di·o·im·mu·no·as·say

(RIA) (rā'dē-ō-im'yū-nō-as'sā)
An immunologic (immunochemical) procedure that uses the competition between radioisotope-labeled antigen (hormone) or other substance and unlabeled antigen for antiserums, resulting in quantitation of the unlabeled antigen; any method for detecting or quantitating antigens or antibodies using radiolabeled reactants.

radioimmunoassay

Any method of measuring the extent of linkage between ANTIGEN and ANTIBODY in which one or other of these is labelled with a radioactive substance (radionuclide). Measurement of radiation can be remarkably precise.

radioimmunoassay

reaction of a radioisotope-labelled agent with specific antibodies; allows light microscopy visualization of antigen-antibody complexes in tissue samples

radioimmunoassay

a sensitive assay method used for the measurement of minute quantities of specific antibodies or any antigen, such as hormones or drugs, against which specific antibodies can be raised; abbreviated RIA. An assay for a specific hormone uses antihormone antibody produced by injecting the human hormone into an animal, such as a rabbit. In the assay either the antigen or antibody is labeled with radioisotope.
References in periodicals archive ?
The action of the pure anti-estrogen is manifested to the same extent with BPA, as demonstrated by immunocyto-chemistry (Figure 4B) and radioimmunoassay (Figure 4C).
The research report categorizes the Europe radioimmunoassay (RIA) market by type into two segments: analyzers and reagents & kits.
Throughout the time when deslorelin acetate implants were present, plasma testosterone concentrations were measured by radioimmunoassay (University of Tennessee Veterinary Medical College, Diagnostic Laboratories, Knoxville, TN, USA), with the exception of 3 individual samples that were measured by radioimmunoassay at a second laboratory (IDEXX Laboratories, Westbrook, ME, USA).
The assay is at least fifty times more sensitive than the gold standard, conventional radioimmunoassay (RIA).
Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma.
Using a newly developed radioimmunoassay, wer measured serum concentrations of leptin in 136 normal-weight subjects and 139 obese subjects (body-mass index, > or = 27.
It's also important to know what type of assay your laboratory performs; radioimmunoassay and high-performance liquid chromatography are the preferred methodologies.
Levels of serum 25(OH)D were measured at baseline by radioimmunoassay.
Arginine vasopressin (AVP) was extracted using previous methods (7) using an AVP Radioimmunoassay kit supplied from Diagnostic Systems Laboratories, Texas, USA.
Each fraction was then characterized and quantified by agarose gel electrophoresis and brevetoxin radioimmunoassay, respectively.
A radioimmunoassay was utilized to determine testosterone titers in those samples.
In conjunction with local and state health departments, an investigation was initiated; as of August 10, a total of 14 passengers had Legionnaires' disease (LD) confirmed by either sputum culture (one patient), detection of antigens of Legionella pneumophila serogroup 1 (Lp1) in urine by radioimmunoassay (seven patients)[2], or fourfold rise in titer of antibodies to Lp1 between acute- and convalescent-phase serum specimens (six patients).