A previous study found quasispecies
compartmentalization in an immunocompromised patient with neurologic manifestations, and its temporal association suggests that neurologic symptoms could be linked to the emergence of neurotropic variants (22).
To gain this understanding we propose to apply whole genome, next generation viral quasispecies
sequencing directly from serial clinical specimens obtained from H5N1-infected patients.
Scientists from Europe and the US discuss coronavirus and rhabdovirus reverse genetics, reverse genetic tools to study the hepatitis C virus, calcivirus genetics, modification of the measles virus and application to pathogenesis studies, bunyavirus reverse genetics and applications to studying interactions with host cells, using reverse genetics to improve influenza vaccines, Bluetongue virus reverse genetics, genetic modification in mammalian orthoreoviruses, and reverse genetics and quasispecies
In an elegant study by Vartanian and colleagues, it was demonstrated that RNA viruses during replication in vitro produce so many different types of species that they must be described in terms of populations of related but distinct genomes called quasispecies
The feasibility of using high resolution genome sequencing of influenza A viruses to detect mixed infections and quasispecies
The detection of the full range of mutant HIV variants in a sample, also known as viral quasispecies
, is expected to be important for management of drug-resistant HIV-1 infections and the choice of combination antiretroviral therapy," explained Martin Daeumer, Study Lead and Laboratory Head of Infectious Disease at the Institute of Immunology and Genetics in Kaiserslautern Germany.
He discuses the origins and mechanisms of RNA viruses, virus quasispecies
, comparative genomics and the macroevolution of viruses, the molecular epidemiology, phylogeography, and emergence of viruses, and applications of these concepts in case studies of viruses like influenza and HIV.
Effect of ribavirin on virus load and quasispecies
distribution in patients infected with hepatitis C virus.
Additionally, the in vivo pressure delivered by a patient's own modified cells led to massive quasispecies
reductions and the production of impaired and less replicative virions.
Genotype and quasispecies
viral heterogeneity, as well as rapid evasion of neutralizing antibodies by this rapidly mutating virus, conspire to render HCV a difficult target for immunopropbylaxis with a vaccine.
Low level of resistance, low viral fitness and absence of resistance mutations in baseline quasispecies
may contribute to high barrier to R1626 resistance in vivo.
Limited data indicate that minority resistant quasispecies
including NNRTI resistance mutations and M184V may affect virological responses .