In the field of infectious disease, NGS is uniquely positioned to support clinical epidemiologic studies by sequencing genomes of infective organisms such as mycoplasma, enabling the quantitation of viral quasi-species
, or monitoring the emergence of drug resistance.
Low levels of 274Y quasi-species in avian influenza A viruses (H5N1) from avian hosts has been reported (11).
Although no experimental data exist that show the growth properties of this resistant variant, examination of the quasi-species population in the cell culture-propagated virus isolate showed that the 274Y variant has become the dominant population.
They are genetically homogenous in the target sequence, unlike many of their natural counterparts, which undergo mutations readily and exist as genetic quasi-species
Alcoholics infected with HCV show greater quasi-species
complexity than do nonalcoholics with HCV infection.
The accurate sequencing of viral genomes, including quasi-species
and other heterogeneous data, requires the capability to detect multiple peaks in any location and distinguish true secondary peaks from noise.
The rest of quasi-species
or the sub-populations remain hidden.