Shifts in the bacterial communities of leachates from three bovine carcasses, sampled at different decomposition times, were investigated by pyrosequencing
of the 16S rRNA.
Detection of antiviral resistance and genetic lineage markers in influenza B virus neuraminidase using pyrosequencing
The two PRR5L CpGs with closest proximity to each other (12 bp apart) were selected for pyrosequencing
analysis (cg00220721 and cg22117805).
The ignored diversity: complex bacteria communities in intensive care units revealed by 16S pyrosequencing
For the pyrosequencing
, the V3-V5 hypervariable region of the 16S rRNA gene was amplified using PCR as previously described (17), with Bact-338F/Bact-909R.
Source LAM resistance M1 2429 1181 mutations detection HBV-RNA Direct YMDD YMDD YVDD sequencing Pyrosequencing
YMDD (94%) YMDD YMDD (30%) YIDD (6%) YVDD (70%) HBV-DNA Direct YMDD YMDD YVDD sequencing Pyrosequencing
YMDD (96%) YMDD YVDD YIDD (4%)
Quantitative polymerase chain reaction and pyrosequencing
assay for long interspersed nuclear element-1 methylation
After amplification, pyrosequencing
was performed using the PyroMark Vacuum Prep Workstation and the PyroMark Q24 instrument (Qiagen) following the manufacturer's instructions.
PCR assays with the validation primers described above for B.
The researcher used pyrosequencing
, a relatively new method of rapidly analyzing bacterial DNA markers, to classify the bacteria into different taxonomic groups.
This is one of the first studies to document such diversity using the pyrosequencing
technique (a large scale DNA sequencing determination technique) on colostrum samples on the one hand, and breast milk on the other, the latter being collected after one and six months of breastfeeding," co-authors, Maria Carmen Collado from the Institute of Agrochemistry and Food Technology (IATA-CSIC) and Alex Mira, researcher at the Higher Public Health Research Centre (CSISP-GVA), said.
DNA was then subjected to 454 pyrosequencing
, a "fingerprinting" technique that provides a snapshot of all the bacterial types present.