sup], In humans and experimental animals, the cornu ammonis 1 (CA1) area in the hippocampus is known as one of the most vulnerable regions to TGCI,[sup], in which pyramidal neurons in the pyramidal layer
die from several days after TGCI.
Additionally, A[beta] deposits (Congo red), GFAP immunoreactivity (immunohisto-chemistry), and neurodegenerative changes in the hippocampal pyramidal layer (Nissl) were examined as measures of [A[beta].
1-42]-provoked degeneration was assessed by measuring the extent of A[beta] deposits (Congo red), GFAP immunoreactivity (immunohistochemistry), and neurodegenerative changes in the hippocampal pyramidal layer (Nissl).
Neurodegeneration in the hippocampal CA1 pyramidal layer
Images of the hippocampal CA1 pyramidal layer (Py) were captured and digitalized (20x) using a Nikon Eclipse E-600 microscope (Tokyo, Japan) coupled to a digital camera, then analyzed using Image Pro Plus Software 6.
flavicollis had a significant asymmetry in the deep subiculum in which the pyramidal layer
is significantly larger in the left hippocampus compared to the right (24).
This feature was limited mainly to the medial dorsal part of the telencephalon, subdivision 2 (Dm2; Figure 2a), and the pyramidal layer
of the mesencephalic stratum griseum central (SGC; Figure 2b), which showed consistent dark axonal processes.
Coagulation with output power of 4-5 W completely damaged the neurons and NF protein in the molecular layer, external granular layer, and external pyramidal layer.
However, when the output power was more than 5 W, the depth of electro-coagulation passed the external pyramidal layer (layer III), reached to the internal granular layer (layer IV), and even the internal pyramidal layer (layer V).
Immunohistochemical analysis showed that the neurons and NF proteins in the molecular layer, external granular layer, and external pyramidal layer were completely damaged in the coagulated cortex.
Caption: Figure 5: Stereological estimate of total microglia (CA1 total) and on CA1 lacunosum molecular (Lac-Mol) and radiatum and pyramidal layers
In this research, nanometric films were created on micrometric pyramidal layers
by using catalytic expulsion characteristics of silver nanoparticles in comparison with silicon, which minimizes the light reflection.