For agarose gel detection, either CHX (cydoheximide) or puromycin
After transformation by lipofection, we performed puromycin
selection for 24 hours, and then returned the transformants to the original culture medium for 48 hours.
Changes in glomerular epithelial cells induced by FGF2 and FGF2 neutralizing antibody in puromycin
A 2-kb fragment of the mouse Cyp1a1 promoter bearing the AHR-responsive enhancer and proximal promoter domains was inserted upstream of the puromycin resistance-eGFP gene complex in the PuroIRESeGFP vector for promoter sorting cell selection, a kind gift from A.
To insure that we tracked only cells positive for a functional AHR, we established the pAHRPuroIRESeGFP cell line, a stable ES cell line that expresses the selection markers puromycin resistance and eGFP under control of the Cyp1a1 promoter, and therefore responds to TCDD treatment (see Supplemental Material, Figure S3A).
HepG2 was highly sensitive to all the anticancer drugs tested including actinomycin D, puromycin, paclitaxel, vinblastine and doxorubicin.
50] values, RA extract almost completely re-sensitized drug resistant HepG2-DR cells to puromycin.
Compared with control, XRCC1-knockdown cells exhibited uniform XRCC1 loss (Figure 2A), although XRCC1 was partially reexpressed within 2 weeks postinfection (Figure 2C), despite being cultured in the presence of the selecting antibiotic puromycin
The relationship between changes in renal gene expression induced by cisplatin, gentamicin, and puromycin with conventional toxicology end points, lesion topography and potential mediators of mechanism of toxicity was also evaluated and are also discussed elsewhere in this mini-monograph (Amin et al.
Three nephrotoxicants were selected for study in male Sprague-Dawley rats, cisplatin, gentamicin, and puromycin (Amin et al.
Key words: biomarkers, cisplatin, gentamicin, microarrays, nephrotoxicity, proximal tubule, puromycin
Using protein synthesis inhibitors such as cycloheximide and puromycin
, investigators have shown that hormone regulated steroid production requires rapid de novo protein synthesis (5).