applied a GC-MS-based platform to measure 272 metabolite peaks in plasma obtained from 52 individuals with heart failure and 57 age-matched controls, and identified pseudouridine as the most significant discriminator of disease status (25).
Serum metabolomics reveals many novel metabolic markers of heart failure, including pseudouridine and 2-oxoglutarate.
Yu and Karijolich studied pseudouridine, a slightly different version of the RNA component uridine.
Pseudouridine is already known to be important for the function of many types of RNA in cells.
The Role Of Pseudouridine
At The Spliceosomal Branch Site: A Molecular Dynamics Analysis.
The purines (except 2-deoxyadenosine), orotic acid, pseudouridine
, uridine, 2-deoxyuridine, 5-hydroxymethyluracil, and thymidine were ionized in negative ionization mode.
Simultaneous determination of purine metabolites, creatinine and pseudouridine
in ruminant urine by reversed-phase high-performance liquid chromatography.
Automated determination of orotic acid, uracil and pseudouridine
in urine by highperformance liquid chromatography with column switching.
Orotic acid, pseudouridine
, 5-hydroxymethyluracil (5-OH-Me-ura), deoxyinosine, deoxyguanosine, and ammonium formate were purchased from Sigma.
Urine from healthy infants contained dominant peaks of uric acid (UA), urea, creatinine, and hippuric acid and usually various amounts of hypoxanthine (HX), xanthine (X), 7-methylguanine, pseudouridine
, and uridine.
GGT complexed to LDL and VLDL), 5'-nucleotidase, leucine aminopeptidase, cholinesterase, copper, iron, ferritin, and pseudouridine
, using previously described procedures (2).
26(s) Oxypurinol-l-riboside Model compound not available Oxypurinol-7-riboside Model compound not available Pseudouridine