These lines can be further utilized for protoplast fusion
, genetic transformation and as a tool for basic studies of plant biochemistry and molecular biology.
increments; Capacitance for 10 500V, 25 3,275 oF; 500 2,500V, 25 and 50 oF; LCD; supply with 1 and 2 mm gap disposable cuvettes (50 each), Chamber for plant protoplast fusion
(one), Electrodes (2mm gap) covering ~90mm petri-dish for plant applications (one), micro-slides for cell/protoplast fusion
1mm gap (10 numbers); with suitable automatic voltage stabilizer.
The use of UV radiation and of protoplast fusion
have resulted in mutant Trichoderma strains, with higher enzyme levels production than parental strains, including chitinases, glucanases and proteases.
have used EMS mutagenesis and interspecific protoplast fusion
to isolate improved probiotic yeasts.
Somatic hybridization by protoplast fusion
is a promising technique for breeding ornamental species and requires reliable in vitro protocols.
giganteus) by protoplast fusion
using polyethylene glycol (PEG).
studies using polyethylene glycol (PEG) is one of the possible alternatives as it is a powerful tool for genetic improvement of this medicinal plant.
Somatic hybrids obtained by protoplast fusion
between Solanum tuberosum L.
breeders use protoplast fusion
to transfer the CMS characteristic to other species as well; for instance, from radish (Raphanus sativus L.
Sreenath and his team hope that, once they have it, they will be able to use protoplast fusion
to induce the gene fragments of fuse with a more commercially viable cultivar and create the first caffeine-free Arabica or Robusta.
It began some 15 years ago, when Gary Harman, a Cornell professor of horticultural science, and Tom Stasz, then a research associate, took two different strains of trichoderma that occur naturally in soil and, using protoplast fusion
, bioengineered a new strain.
Using a mix of conventional and advanced breeding techniques -- such as anther culture, tissue culture, hybrid sorting, and protoplast fusion
-- B&W eventually produced Y-1.