protoplast fusion


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protoplast fusion

the joining together of two or more PROTOPLASTS. Often the presence of polyethylene glycol or electric fields will enhance the frequency of fusion. In the fusion unit the DNA from the component cells may undergo RECOMBINATION. This method is useful in GENETIC ENGINEERING. It may allow the exchange of DNA between unrelated ORGANISMS if they are capable of undergoing protoplast fusion.
References in periodicals archive ?
These lines can be further utilized for protoplast fusion, genetic transformation and as a tool for basic studies of plant biochemistry and molecular biology.
increments; Capacitance for 10 500V, 25 3,275 oF; 500 2,500V, 25 and 50 oF; LCD; supply with 1 and 2 mm gap disposable cuvettes (50 each), Chamber for plant protoplast fusion (one), Electrodes (2mm gap) covering ~90mm petri-dish for plant applications (one), micro-slides for cell/protoplast fusion 1mm gap (10 numbers); with suitable automatic voltage stabilizer.
have used EMS mutagenesis and interspecific protoplast fusion to isolate improved probiotic yeasts.
Somatic hybridization by protoplast fusion is a promising technique for breeding ornamental species and requires reliable in vitro protocols.
giganteus) by protoplast fusion using polyethylene glycol (PEG).
Protoplast fusion studies using polyethylene glycol (PEG) is one of the possible alternatives as it is a powerful tool for genetic improvement of this medicinal plant.
breeders use protoplast fusion to transfer the CMS characteristic to other species as well; for instance, from radish (Raphanus sativus L.
Sreenath and his team hope that, once they have it, they will be able to use protoplast fusion to induce the gene fragments of fuse with a more commercially viable cultivar and create the first caffeine-free Arabica or Robusta.
It began some 15 years ago, when Gary Harman, a Cornell professor of horticultural science, and Tom Stasz, then a research associate, took two different strains of trichoderma that occur naturally in soil and, using protoplast fusion, bioengineered a new strain.
Using a mix of conventional and advanced breeding techniques -- such as anther culture, tissue culture, hybrid sorting, and protoplast fusion -- B&W eventually produced Y-1.