differ between tissues and between biological states.
Recently, use of mass spectrumetrybased (MS) proteomics technology has further contributed to the identification of the proteome
that make up spermatozoa (6-12).
The authors highlight the comparative higher complexity of functioning proteins, the difficulty of predicting post-translational processing from mRNA sequence "alone, and the frequent disparity between mRNA levels and protein expression, suggesting that only direct analysis of the proteome
itself can characterize proteins with certainty.
It's called tissue proteome
profiling, and it appears to be a powerful new tool for both diagnosis and prognosis.
Protometrix provides researchers access to functional protein content representing vast portions of the proteomes
of pharmaceutically relevant organisms, such as human, mouse, rat, dog and yeast, in a high productivity microarray format.
Another analysis can be performed by kinetically profiling protein Changes in the serum proteome
over the course of pretoxic, toxic, and recovery stages of chemical exposure.
The Human Proteome
Organization, or HUPO, was formed to promote research and large-scale analysis of the human proteome
That absolutely requires proteome
fractionation on the protein level, not only on the peptide level.
On completion of the production program, the Chemical Printer will become a key Proteomics Alliance (Shimadzu Biotech, Proteome
Systems and Sigma-Aldrich) instrument which will be integrated into Proteome
Systems' ProteomIQ(TM) platform for high-throughput Proteomics.
That absolutely requires the proteome
fractionation on the protein level and not only on the peptide level.
Its technology allows definition and study of proteomes
(the comprehensive complement of proteins produced by an organism) of any species for the first time.
The proteomic scientist is faced with the daunting challenge of intelligently interrogating proteomes
for high impact biological and pharmaceutical discovery.