proteolytic enzyme


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enzyme

 [en´zīm]
any protein that acts as a catalyst, increasing the rate at which a chemical reaction occurs. The human body probably contains about 10,000 different enzymes. At body temperature, very few biochemical reactions proceed at a significant rate without the presence of an enzyme. Like all catalysts, an enzyme does not control the direction of the reaction; it increases the rates of the forward and reverse reactions proportionally.

Enzymes work by binding molecules so that they are held in a particular geometric configuration that allows the reaction to occur. Enzymes are very specific; few molecules closely fit the binding site. Each enzyme catalyzes a specific type of chemical reaction between a few closely related compounds, which are called substrates of the enzyme.

Enzymes are given names ending in -ase. In older names, the suffix is added to the name of the substrate, as in amylase, an enzyme that breaks down the polysaccharide amylose. In newer names, the suffix is added to the type of reaction, as in lactate dehydrogenase, an enzyme that converts lactate to pyruvate by transferring a hydrogen atom to nicotinamide-adenine dinucleotide (NAD).
Regulation of Enzymes. The reaction rate of an enzyme-catalyzed reaction varies with the pH, temperature, and substrate concentration. Under physiologic conditions the rates of many reactions are controlled by substrate concentrations. Certain key reactions are controlled by one of three different mechanisms.

In allosteric regulation, the enzyme can bind molecules, which are referred to as effectors, at a site other than the active site, which is referred to as an allosteric site. In many biochemical pathways the enzyme that catalyzes the first reaction in the pathway is inhibited by the final product of the last reaction, so that when sufficient product is present the whole pathway is shut down. This is an example of negative feedback.

Many enzymes are regulated by phosphorylation. A phosphate group is attached to the enzyme by another enzyme, called a protein kinase. When the enzyme is phosphorylated it changes its shape and thus its activity. Phosphorylation activates some enzymes and inactivates others; by this means one protein kinase can control several enzymes.

All enzymes are controlled by their rate of synthesis. Like all proteins, enzymes are synthesized by ribosomes, which translate the genetic information coded in the deoxyribonucleic acid (DNA) of the chromosomes into the specific amino acid sequence of the enzyme. The expression of many genes is controlled by the processes of genetic regulation. Thus, although each cell contains the information to make all of the body's enzymes, it actually makes only those appropriate for its specific type of cell. The synthesis of some enzymes can be induced or repressed by the action of specific hormones, substrates, or products so that the enzyme is produced only when metabolic conditions require its presence.
Inborn Errors of Metabolism. Hundreds of genetic diseases that result from deficiency of a single enzyme are now known. Many of these diseases fall into two large classes. The aminoacidopathies result from deficiency of an enzyme in the major pathway for the metabolism of a specific amino acid. The amino acid accumulates in the blood, and it or its metabolites are excreted in the urine. The lysosomal storage diseases result from deficiency of a lysosomal enzyme and the accumulation of the substance degraded by that enzyme in lysosomes of cells throughout the body. The stored material is usually a complex substance, such as glycogen, a sphingolipid, or a mucopolysaccharide.ƒ

An example of an aminoacidopathy is phenylketonuria (PKU), which results from a deficiency of the enzyme phenylalanine hydroxylase, which converts the amino acid phenylalanine to tyrosine. Phenylalanine accumulates in the blood and phenylpyruvic acid is excreted in the urine. The phenylalaninemia eventually results in mental retardation due to defective formulation of myelin. However, PKU can be detected at birth by a screening test for phenylalanine in the blood, and clinical symptoms can be avoided by strict adherence to a low-phenylalanine diet.

An example of a lysosomal storage disease is tay-sachs disease, which results from a deficiency of the enzyme hexosaminidase A. The stored substance is a sphingolipid, GM2-ganglioside, which accumulates in nerve tissue, causing blindness and mental deterioration. No cure is possible, but antenatal diagnosis can be made by determining hexosaminidase A activity in fetal fibroblasts from an amniotic fluid specimen drawn by amniocentesis. It is also possible to identify carriers (heterozygotes) who are at risk for having children with the disease.
Enzyme Assays. Several enzymes are important in clinical pathology. Enzymes characteristic of a tissue are released into the blood when the tissue is damaged; hence assays of serum enzyme levels can aid in the diagnosis or monitoring of specific diseases. Lipase and amylase levels are useful in pancreatic diseases; alkaline phosphatase (ALP), lactate dehydrogenase (LD), aspartate transaminase (AST or GOT), and alanine transaminase (ALT or GPT) in liver diseases; and LD and creatine kinase (CK) in myocardial infarction. ALP is also released in bone diseases. Many enzymes have different forms (isoenzymes) in different organs. The isoenzymes can be separated by electrophoresis in order to determine the origin of the enzyme. Isoenzymes of LD, CK, and ALP have the most clinical utility.
See accompanying table.
activating enzyme one that activates a given amino acid by attaching it to the corresponding transfer ribonucleic acid.
brancher enzyme (branching enzyme) α-glucan-branching glycosyltransferase: an enzyme involved in conversion of amylose to amylopectin; deficiency of this enzyme causes amylopectinosis.
constitutive enzyme one produced by a microorganism regardless of the presence or absence of the specific substrate acted upon.
debrancher enzyme (debranching enzyme) dextrin-1,6-glucosidase: an enzyme that acts on glucose residues of the glycogen molecule and is important in glycogenolysis; deficiency of this enzyme causes Forbes' disease.
induced enzyme (inducible enzyme) one whose production requires or is stimulated by a specific small molecule, the inducer, which is the substrate of the enzyme or a compound structurally related to it.
proteolytic enzyme one that catalyzes the hydrolysis of proteins and various split products of proteins, the final product being small peptides and amino acids.
repressible enzyme one whose rate of production is decreased as the concentration of certain metabolites is increased.
respiratory e's enzymes of the mitochondria, e.g., cytochrome oxidase, which serve as catalysts for cellular oxidations.

proteolytic enzyme

An enzyme that catalyzes the conversion of proteins into peptides.
See also: enzyme

proteolytic enzyme

any enzyme concerned with PROTEOLYSIS. see ENDOPEPTIDASE, EXOPEPTIDASE.

enzyme

any protein that acts as a catalyst, increasing the rate at which a chemical reaction occurs. The animal body probably contains about 10,000 different enzymes. At body temperature, very few biochemical reactions proceed at a significant rate without the presence of an enzyme. Like all catalysts, an enzyme does not control the direction of the reaction; it increases the rates of the forward and reverse reactions proportionally.

activating enzyme
one that activates a given amino acid by attaching it to the corresponding transfer ribonucleic acid.
enzyme assays
several enzymes are important in clinical pathology. Enzymes characteristic of a tissue are released into the blood when the tissue is damaged, and enzyme levels in the blood can aid in the diagnosis or monitoring of specific diseases. Lipase and amylase levels are useful in pancreatic diseases; alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in liver diseases; and lactate dehydrogenase (LD), AST and creatine kinase (CK) in muscle disease. ALP is also released in bone diseases. Many enzymes have different forms (isoenzymes) in different organs. The isoenzymes can be separated by electrophoresis in order to determine the origin of the enzyme. Isoenzymes of LD, CK and ALP have the most clinical utility.
brancher enzyme, branching enzyme
amylo-(1,4→1,6)-transglycosylase; important in the synthesis of the branched glycogen molecule. Absence of the enzyme causes an increase in the length of the glucose chains and a decrease in the number of branch points in the glycogen molecules.
congenital enzyme deficiency
in humans hundreds of genetic diseases that result from deficiency of a single enzyme are now known. Many of these diseases fall into two large classes. The aminoacidopathies, e.g. phenylketonuria (PKU), result from deficiency of an enzyme in the major pathway for the metabolism of a specific amino acid. The amino acid accumulates in the blood, and it or its metabolites are excreted in the urine. The lysomal storage diseases, e.g. gangliosidosis, mannosidosis, result from deficiency of a lysomal enzyme and the accumulation of the substance degraded by that enzyme in lysosomes of cells throughout the body. The stored material is usually a complex substance, such as glycogen, a sphingolipid or a mucopolysaccharide. Many similar diseases are now identified in animals and are to be found under the specific name of each disease.
constitutive enzyme
one produced by a microorganism regardless of the presence or absence of the specific substrate acted upon.
core enzyme
the smallest aggregate of an enzyme's subunits that has enzymatic activity.
debrancher enzyme, debranching enzyme
dextrin-1,6-glucosidase: an enzyme that acts to move glucose residues of the glycogen molecule, and is important in glycogenolysis.
induced enzyme, inducible enzyme
one whose production requires or is stimulated by a specific small molecule, the inducer, which is the substrate of the enzyme or a compound structurally related to it.
enzyme induction
the effect some compounds such as phenobarbitone and phenytoin have in increasing the activity of microsomal hepatic enzymes. This may cause alterations in the metabolism of concurrently administered drugs.
microsomal e's
those associated with the endoplasmic reticulum of cells, particularly of the liver.
proteolytic enzyme
one that catalyzes the hydrolysis of proteins and various split products of proteins, the final product being small peptides and amino acids.
repressible enzyme
one whose rate of production is decreased as the concentration of certain metabolites is increased.
respiratory e's
enzymes of the mitochondria, e.g. cytochrome oxidase, which serve as catalysts for cellular oxidations.
restriction e's

proteolytic

1. pertaining to, characterized by, or promoting proteolysis.
2. a proteolytic enzyme.

proteolytic enzyme
includes pepsin from the stomach; trypsin, chymotrypsin, elastase, carboxypeptidase A and B, ribonuclease and deoxyribonuclease—all from the exocrine pancreas; enteropeptidase, aminopeptidase, dipeptidase, nuclease, nucleotidases and nucleosidases—all from the intestinal mucosa.
References in periodicals archive ?
Proteolytic enzymes break down excess fibrin that has been linked to chronic systemic inflammation which feeds chronic diseases, including cancer.
Influence of molting and starvation on the synthesis of proteolytic enzymes in the midgut gland of the white shrimp Penaeus vannamei.
Ageing, therefore, refers to holding of meat at refrigerated temperatures for an extended period to allow natural enzymatic reactions to take place to enhance tenderness and flavour, thus, allowing proteolytic enzymes to break down some of the complex proteins contained in the muscle [18,19].
Several proprietary formulations of bromelain are available, some of which also contain other proteolytic enzymes such as trypsin, chymotrypsin, and pancreatin.
This high protein content could be broken down by proteolytic enzymes in the digestive tract of abalone, even though abalone are considered to be herbivorous animals that feed on mainly macroalgae, which are richer in carbohydrates.
Actazin has a unique, natural composition of gut health supporting nutrients, including a standardized level of actinidin, a proteolytic enzyme unique to kiwi fruit, insoluble and soluble fiber, prebiotics and high level of polyphenols.
1934, "Further Studies on the proteolytic enzyme content of latex from the Fig and related trees," J.
Maastricht, Netherlands) has patented a process for the assessment of an active proteolytic enzyme in blood or other biological fluid sample possibly comprising a complex of said proteolytic enzyme and .
An open label study to determine the effects of an oral proteolytic enzyme system on whey protein concentrate metabolism in healthy males.
Metabolized animal protein leaves an ash after all food value is removed, and this ash is acid-producing, further disabling the little proteolytic enzyme that remains.
Arginine provides a specific locus for the most aggressive proteolytic enzyme, trypsin.
SEB), Chino, CA, pioneered the production of papain, a proteolytic enzyme derived from papaya.