protein truncation test

protein truncation test

a method for detection of one or more translation termination mutations in a gene that cause a truncated, usually inactive, protein to be synthesized. The appropriate genomic DNA or mRNA is isolated, amplified by polymerase chain reaction, and used as a template for in vitro transcription and translation. The size of the resulting protein is compared with that of a wild type protein by means of SDS-polyacrylamide gel electrophoresis.
References in periodicals archive ?
If the common mutations were excluded and there was a family history of breast cancer, the laboratory would add a protein truncation test for exon 11 of the BRCA1 gene and exon 10 and 11 of the BRCA2 gene.
Of 131 protein truncation tests performed, 125 were prior to 2010, and 80% of the probands went on to have a protein truncation test (125/157 probands who tested negative for common mutations).
The most widely available FAP genetic test is protein truncation test or PTT.
We verified the presence of the E244X mutation in POMC with the in vitro protein truncation test by adding 5 [micro]L of PCR product and 1 [micro]L of [3H]leucine to a reticulocyte lysate (Promega) under the manufacturer's recommended reaction conditions.
After retrotranscription and translation of this mutated DNA, we verified by the protein truncation test that this variant caused the synthesis of a truncated peptide shorter (~22.
The digital protein truncation test used in this study is not yet a clinically useful screening test.
As the list of known heritable gene disorders grows, advanced research techniques such as PCR reverse dot blot and the protein truncation test are bolstering the role of molecular biology in genetic testing and counseling.
Rather than describing many different point mutations strategies, den Dunnen has elected to cover specifically the protein truncation test.
Commonly used screening methods include denaturing gradient gel electrophoresis (DGGE) (5), temperature gradient gel electrophoresis (TGGE) (6), singlestranded conformation polymorphism, heteroduplex analysis (HA), chemical mismatch cleavage, enzyme mismatch cleavage (EMC), the protein truncation test, mismatch-binding protein, and cleavase fragment length polymorphism.
Many methods have been reported for the study of BRCA mutations, including allele-specific oligonucleotide hybridization (8, 9), allele-specific PCR (10), PCR-mediated site-directed mutagenesis (11,12), heteroduplex analysis (HDA) (13-15), single-strand conformation polymorphism (14,16), and the protein truncation test (14,15).
Methods such as single-strand conformation polymorphism, denaturing gradient-gel electrophoresis, or the protein truncation test have proven useful for scanning coding regions and critical intron sequences for sequence variants in a variety of disorders [24-26].
Moreover, in a disease-specific manner, the text addresses exactly how to perform molecular diagnostic testing, often delving into the nitty gritty of each assay, and encompasses techniques ranging from allele-specific oligonucleotide hybridization to protein truncation tests.
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