The development of protein chip
or microarray technology has provided a highly sensitive, high-throughput method for disease diagnosis (8, 9) by facilitating detection--in small amounts of patient samples--of antibodies to antigens from infectious viruses or bacteria (10, 11).
In their efforts to identify suitable biomarkers of the progression from normal cognitive functioning to mild cognitive impairment and AD, researchers have begun to use protein chip
arrays whose surfaces have been treated chemically or biochemically so that the arrays can bind only proteins with certain structural or functional characteristics.
Another quantitative MS method is the surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry, which has a quantitative protein chip
and it is known as SELDI-TOF-MS-based Protein Chip
When a liquid such as plasma is run over a protein chip
, the antibodies bind their target protein, revealing its presence.
Hayward, CA; 206-463-4800) announced that the United States Patent and Trademark Office has issued a broad patent covering a fundamental protein chip
technology developed by Peter Wagner, Ph.
2]O) was added, vortex-mixed (30 s), left 10 min on ice, centrifuged, and then 2 [micro]L of the supernatant was pipetted onto the protein chip
There are also methods for protein expression profiling, identifying protein- protein interactions, and protein chip
company Ciphergen chose the conference to introduce its new SEND ProteinChip Arrays, which incorporate the matrix into the array's surface chemistry, resulting in lower chemical noise.
Ann Arbor, MI; 734-975-4800) announced that its internal proteomics research and development team has successfully validated the use of its GeneTAC HybStation, G3, LS IV and UC4 instruments for protein chip
production, processing, imaging and analysis.
Thin-tissue cryostat sections (8 /,m) were deposited directly on the different protein chip
surfaces (Ciphergen Biosystems).
The company has sold over 350 Protein Chip
systems as of December 2002, with approximately 55% of its customers in the pharmaceutical industry and 45% in academia.
A protein chip
is a chemically modified surface onto which a high density of protein-based probes have been precisely applied in an ordered manner.