protein chip

protein chip

A tool for evaluating very large numbers of proteins (e.g., the entire proteome of an organism) using DNA microarray technology. Uses include the evaluation of interactions between proteins and other molecules; the development of new drugs; and the diagnosis of diseases, such as immunological disorders, in which small concentrations of abnormal proteins or antigens occur in body fluids.

protein chip

an orderly arrangement of proteins, immobilized as spots on a matrix at precise locations, to form an array, analogous to DNA CHIP/MICROARRAY. Protein chips can be fabricated by robotically depositing proteins/antibodies on to the surface of a microscope slide. A labelled (see LABEL protein sample, which may contain many analyte proteins, can be applied to the chip. After incubation sample proteins that bind to their capture proteins on the chip can be detected and identified by locating the position/intensity of the label, for example fluorescence. A protein chip allows thousands of proteins to be studied simultaneously. It can be used, for example, for profiling proteins in cells under different conditions and for studying protein-protein and protein-small molecule interactions (see PROTEOMICS).
References in periodicals archive ?
The development of protein chip or microarray technology has provided a highly sensitive, high-throughput method for disease diagnosis (8, 9) by facilitating detection--in small amounts of patient samples--of antibodies to antigens from infectious viruses or bacteria (10, 11).
In their efforts to identify suitable biomarkers of the progression from normal cognitive functioning to mild cognitive impairment and AD, researchers have begun to use protein chip arrays whose surfaces have been treated chemically or biochemically so that the arrays can bind only proteins with certain structural or functional characteristics.
Another quantitative MS method is the surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry, which has a quantitative protein chip and it is known as SELDI-TOF-MS-based Protein Chip[R].
When a liquid such as plasma is run over a protein chip, the antibodies bind their target protein, revealing its presence.
Hayward, CA; 206-463-4800) announced that the United States Patent and Trademark Office has issued a broad patent covering a fundamental protein chip technology developed by Peter Wagner, Ph.
2]O) was added, vortex-mixed (30 s), left 10 min on ice, centrifuged, and then 2 [micro]L of the supernatant was pipetted onto the protein chip.
There are also methods for protein expression profiling, identifying protein- protein interactions, and protein chip technology.
Protein chip company Ciphergen chose the conference to introduce its new SEND ProteinChip Arrays, which incorporate the matrix into the array's surface chemistry, resulting in lower chemical noise.
Ann Arbor, MI; 734-975-4800) announced that its internal proteomics research and development team has successfully validated the use of its GeneTAC HybStation, G3, LS IV and UC4 instruments for protein chip production, processing, imaging and analysis.
Thin-tissue cryostat sections (8 /,m) were deposited directly on the different protein chip surfaces (Ciphergen Biosystems).
The company has sold over 350 Protein Chip systems as of December 2002, with approximately 55% of its customers in the pharmaceutical industry and 45% in academia.
A protein chip is a chemically modified surface onto which a high density of protein-based probes have been precisely applied in an ordered manner.