Recently, the CPC acylase is very attractive at industrial level because it possesses very simple with reducing cost, although its catalytic performance, including its activity and product inhibition resistance, is not yet as satisfactory as that of the GL-7-ACA acylase (2,11).
The natural of cephalosporin C acylase catalyzed the CPC to 7-ACA directly in a very low efficiency (14,15,16), significant substrate inhibition (14), and product inhibition (14).
Furthermore, we will develop a RNA-promoted reaction with turnover beyond product inhibition
Proposed model describes the reaction kinetics for the production of [beta]-CD and [gamma]-CD, considering substrate and product inhibition
, reversibility of the reaction and considering that the cyclization reaction catalyzed by CGTase produces simultaneously the two CDs.
Production of protease by fermentation in homogeneous system inhibited by product inhibition and catabolic repression as cells and product formation takes place in a single phase .
The major drawback of protease production in industrial scale is catabolic repression and product inhibition, which can be solved by doing extractive fermentation using ATPSs.
The apparent kinetic parameters of this equation, reflecting also the phenomenon of product inhibition
by ATP excess , have the following meaning:
Pervaporation can be used for the in situ separation of a product in order to increase the time and rate of the fermentation process, as well as to avoid product inhibition
NATURAL PRODUCT INHIBITION
STUDIES WITH NAD SYNTHETASE.