primer


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prim·er

(prī'mĕr),
1. A molecule, which may be a small polymer, that initiates the synthesis of a larger structure. Synonym(s): starter
2. A pheromone that causes a long-term physiologic change.

primer

(prī′mər)
n.
Genetics A segment of DNA or RNA that is complementary to a given DNA sequence and that is needed to initiate replication by DNA polymerase.

primer

[prī′mər]
1 a short piece of DNA or RNA complementary to a given DNA sequence that acts as a point at which replication can proceed, as in a polymerase chain reaction.
2 a molecule, such as a small polymer, that induces the synthesis of a larger molecule.

primer

1. A short RNA sequence paired with one DNA strand that provides a free 3’-OH end on which synthesis of a deoxyribonycleotide chain can start.
2. The short nucleotide sequence used to start the POLYMERASE CHAIN REACTION (PCR) process.

primer

a short OLIGONUCLEOTIDE, which is required for the functioning of various POLYMERASES. In DNA REPLICATION, the primer is a short RNA sequence that anneals to the DNA TEMPLATE and is extended by DNA POLYMERASE. In the POLYMERASE CHAIN REACTION, the primer is a short oligonucleotide that initiates the PCR. In OLIGONUCLEOTIDE-DIRECTED MUTAGENESIS, the synthetic oligonucleotide used to introduce the mutation is a mutagenic primer that initiates DNA synthesis.

primer

oligonucleotide which is hydrogen-bonded to the template strand of DNA; required for the replication of DNA by polymerase.

random primer method
a method of labeling DNA in vitro.
primer walking
an orderly method for sequencing DNA which involves obtaining some sequence, then making an oligonucleotide primer to the end of the sequence obtained and using it as a primer for the next sequencing reaction and repeating this until the whole sequence is determined.
References in periodicals archive ?
This would lead to a false positive signal in the LCR reaction, as this one primer ligation product is now capable of amplifying normally.
There is no exposure during mixing and no need for disposal of liquid primer after mixing.
This simulation allowed us to assess whether the positional effect of a mismatch could be extrapolated to other primer locations and thus be used to predict how many simultaneous mismatches could be tolerated in a qPCR reaction.
Primers are mysterious things which I fear we handloaders take for granted.
Students can calculate the optimal annealing temperature on the basis of primer compositions and design an experiment to test different temperature ranges in order to determine the optimal annealing temperature.
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published HRV-specific PCR primer pairs (most of which were first published before HRV-C was reported), independent of most variables described above, by testing a panel of 57 clinical specimen nucleic acid extracts from combined nose and throat swabs from preschool children with colds and influenza-like illnesses in Melbourne, Australia.
The metal side of the blister shows the same chemistry as the adhesive, leading to the conclusion that the particle of primer and adhesive was deposited on the surface of the coated adhesive part prior to bonding with rubber.
Written by leading experts, the primer papers provide background information on key topics in a non-technical format.
A 6th generation product has two steps (the same as 5th), but instead of the primer and bonding agent mixed together, the etch and primer are mixed together.
A new SMC primer from Red Spot Paint & Varnish Co.
Exploring the cares for the pregnant mare, assistance at birth, coping with the common problems of newborn colts, what to expect in the first days and weeks, nutrition and the foal, early handling and training, first hoof care, grooming and bath, vaccinations and veterinary care, and weaning the foal, The Foaling Primer is a dependable and comprehensive month-to-month guide to proper care of a baby horse.
A chromate-inhibited primer has been characterized by Doppler broadening energy spectroscopy (DBES), scanning electron microscopy (SEM), and Raman spectroscopy.