reverse genetics

(redirected from positional cloning)
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reverse genetics

a conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism.
Synonym(s): positional cloning

reverse genetics

methods such as antisense nucleic acids and site-directed mutagenesis that are used to selectively study gene function. Contrasts with classical genetics which depends on the isolation and analysis of cells (animals) with random mutations that can be identified.
References in periodicals archive ?
Possible methodologies include, but are not limited to, traditional linkage analysis, sibling pair and affected-pedigree-member methods, case-control or family-based association studies, linkage disequilibrium mapping in genetically isolated populations, candidate gene analysis, cytogenetic studies to identify chromosomal abnormalities associated with a disorder, and positional cloning.
Positional cloning of the Chediak-Higashi syndrome gene: genetic mapping of the beige locus on mouse chromosome 13.
Positional cloning of the mouse obese gene and its human homologue.
Covered methods--adaptable to studying the genetic components of other conditions--include bioinformatics, cytogenetic analyses, mutation screening, microarray analysis, positional cloning, and recording and mapping of cardiac physiology.
The COB1 gene was discovered utilizing a novel proprietary technology developed by Myriad's geneticists to advance traditional positional cloning, the technique used in the 1990s to discover genes such as BRCA1 and BRCA2 for breast cancer and ovarian cancer.
Fine structure mapping is needed for positional cloning of the scs gene leading to the detailed characterization of nuclear-cytoplasmic interaction in wheat.
This capability is critical in both positional cloning of disease genes and drug discovery through Myriad's ProNet protein interaction technology.
The completion of human and other genome sequencing, along with other new technologies, such as mutation analysis and microarray, has dramatically accelerated the progress in positional cloning of genes from mutated models.
The researchers identified a mouse phenotype that displayed progressive loss of muscle tone and locomotor ability, in a similar fashion to the ALS disease progression in humans, and the research groups performed positional cloning to locate the mutated gene responsible.
As we all know, a massive frontal attack on both human and non-human genomes is currently underway, Positional cloning, subtractive hybridization, comparative genomic hybridization, expression profiling, high-throughput screening techniques, and protein and other expression systems are all creating massive quantities of data in areas related to structural genomics, functional genomics, and proteomics, Bioinformation systems are therefore rapidly being developed to help integrate and effectively mine this massive amount of information.
The patented methodology is used by the company in gene discovery, both in traditional positional cloning projects and in its ProNet protein-interaction technology.

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