Joe River and since the quality of their polytene
chromosomes was excellent, I compared chromosome diversity within and among samples taken in 2011, 2012 and 2013 (avg.
Only a few basal cells contained polytene
We used conventional methods of collection (Shields and Procunier, 1982) and identification of larvae (Currie, 1986), the Feulgen method of Rothfels and Dunbar (1953) to stain polytene
chromosomes and gonads of larvae and the standard chromosome maps for the Simulium arcticum complex (Shields and Procunier, 1982) to determine chromosomal variation.
The genome of the olive fruit fly Bactrocera oleae: localization of molecular markers by in situ hybridization to the salivary gland polytene
Its chromosomal phylogeny has been established by comparing the banding patterns of the various species and also by the observation of the polytene
chromosomes in many interspecific hybrids (Ruiz and Wasserman 1993).
Due to commercial activity in the area, one can witness heaps of waste including polytene
bags, bottles and even construction waste at a number of places.
By examination of the polytene
chromosome banding pattern of the larvae, it was determined that these midges are Chironomus columbiensis Wulker, Sublette, Morath, and Martin.
The simulans clade species are homosequential at the level of polytene
chromosomes and differ from melanogaster only by one large inversion on the right arm of the third chromosome and a few very small rearrangements (Lemeunier and Ashburner 1976).
Almost 60 years have elapsed since the discovery of variation in the polytene
chromosomes of Drosophila (Sturtevant and Dobzhansky 1936).
chromosomes of third instar larvae were stained and squashed in lacto-aceto-orcein solution.
The genotypes of adult females and their mates are determined by cytological examination of polytene
chromosomes from 12 offspring.
The major "loci" in this case are the ribosomal DNA (rDNA) multigene family complex on the X chromosome and a second X-linked locus that controls the pattern of somatic underreplication of rDNA in polytene