To quantify the proportion of ribosomes in the monoribosome (monosome) and polyribosome
(polysome) fractions, sucrose gradients with an EDTA-containing cushion were used to accumulate all of the different size classes of polysomes (measured as one large peak).
FMRP associates with polyribosomes
as an mRNP, and the 1304N mutation of severe fragile X syndrome abolishes this association.
Transmission electron microscopy revealed disorganized endoplasmic reticulum, dispersion of the polyribosomes
, a variety of mitochondrial changes, and glycogen redistribution.
Most studies to date indicate that RNAi occurs in the cytoplasm by acting on translating polyribosomes
Additional features of the tumor cells included moderately abundant mitochondria, stacks of rough endoplasmic reticulum, and polyribosomes
Mercury compounds can bind to the RNA of tobacco mosaic viruses (Katz & Santilli, 1962), several synthetic polyribosomes
, and yeast soluble RNA (Kawade, 1963).
The cytoplasm contained many mitochondria, prominent Golgi apparatus, numerous polyribosomes
, moderate endoplasmic reticulum, a few lipid droplets, and, focally, intermediate filaments.
Ribosomes and polyribosomes
are present in the squid giant axon: an immunocytochemical study.
As this technique is supposed to stain all the neurons because of the affinity of methylene blue by polyribosomes
and all the neurons, regardless of physiologic features, have this organelle, we would then have the total number of neurons.
The exact percentage of glassy cells needed to make the diagnosis of GCC is not well established, but it may range from 30% to 100%[1,9] Ultrastructurally, glassy cells may show intermediate filaments, polyribosomes
, tonofibrils, and desmosomes.
Electron microscopy revealed cells with prominent nucleoli and abundant cytoplasmic polyribosomes
Those cells had abundant simple cytoplasm, which included a few mitochondria, a small Golgi apparatus, and multiple free polyribosomes