16S rDNA sequencing: DNA was isolated from selected bacterial strains varying in polygalacturonase
activity by CTAB (Cetyltrimethylammonium bromide) method (Ausubel et al.
Firstly, pectinase is not a single enzyme, but, rather, a set of several pectin-splitting substances: pectinesterases, polygalacturonases
, pectin lyases, and pectate lyases.
activity was significantly affected by the maturation degree of the fruits; it increased gradually until achieving its maximum value in stage 4 (TP).
They prepared the purees using a commercial polygalacturonase
and a highly purified pectinesterase.
Plant cell wall-degrading enzymes including polygalacturonase
(PG) allow Xf to break cell walls and spread within the grapevine.
oxytoca pehX 344 20 Peh-D polygalacturonase
gene eaeA-F EHEC/EPEC E.
inhibiting proteins in plant fleshy fruits during their ripening and infections.
Optimizing grape rootstock production and export of inhibitors of Xylella fastidiosa polygalacturonase
The effects of low-dose ultraviolet light-C treatment on polygalacturonase
activity, delay ripening and Rhizopus soft rot development of tomatoes.
Among the depolymerases, polygalacturonase
is the major enzyme with a hydrolytic function and lyases (or transeleminases) which cleaves glycosidic bonds forming unsaturated product[DELTA](4,5-D-galacturonate) through transelimination reaction .
2004) Compound Enzyme Fungal Type Cellulose Cellulase ERM, ECM Cellobiohydrolase ERM, ECM Hemicellulose Xylanase ERM, ECM Mannosidasc ERM Galactosidase ERM Arabinosidase ERM Glucanasc ERM Pectin Polygalacturonase
ERM, ECM Tannins Polyphenol oxidase ERM, ECM Peroxidase ERM, ECM Catcchol oxidase ERM, ECM Tyrosinase ERM, ECM Lactase ERM, ECM Lignin Manganese peroxidases ECM Lignase ERM Proteins Acid protease ERM, ECM Chitin Chifnase ERM, ECM Lipids Fatty acid esterase ECM
Barrett, Thermal inactivation of pectin methylesterase, polygalacturonase
, and peroxidase in tomato juice, Journal of Agricultural and Food Chemistry, 50(21), 6153-6159 (2002).