polycloning site

polycloning site

in recombinant DNA technology, an artificially synthesized nucleotide sequence incorporated in a plasmid that contains multiple cleavage sites for different restriction enzymes enabling a choice of the most appropriate restriction enzyme for cloning. Called also restriction site bank or polylinker site.
References in periodicals archive ?
3 kb EcoRI-NheI DNA fragment was gel purified and ligated into the polycloning site of the expression vector, pJY505, which is upstream and in frame of the lacZ gene that encodes [beta] galactosidase.